ID A0A0C9NTT4_9BACT Unreviewed; 486 AA.
AC A0A0C9NTT4;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:GAN35290.1};
GN ORFNames=BROSI_A3839 {ECO:0000313|EMBL:GAN35290.1};
OS Candidatus Brocadia sinica JPN1.
OC Bacteria; Planctomycetota; Candidatus Brocadiia; Candidatus Brocadiales;
OC Candidatus Brocadiaceae; Brocadia.
OX NCBI_TaxID=1197129 {ECO:0000313|EMBL:GAN35290.1, ECO:0000313|Proteomes:UP000032309};
RN [1] {ECO:0000313|EMBL:GAN35290.1, ECO:0000313|Proteomes:UP000032309}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JPN1 {ECO:0000313|EMBL:GAN35290.1,
RC ECO:0000313|Proteomes:UP000032309};
RA Oshiki M., Shinyako-Hata K., Satoh H., Okabe S.;
RT "Draft Genome Sequence of an Anaerobic Ammonium-Oxidizing Bacterium,
RT "Candidatus Brocadia sinica".";
RL Genome Announc. 3:e00267-15(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAN35290.1}.
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DR EMBL; BAFN01000001; GAN35290.1; -; Genomic_DNA.
DR RefSeq; WP_052565423.1; NZ_BAFN01000001.1.
DR AlphaFoldDB; A0A0C9NTT4; -.
DR OrthoDB; 226469at2; -.
DR Proteomes; UP000032309; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00415; ladderane_biosynthesis; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000032309}.
FT DOMAIN 5..139
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 164..394
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 486 AA; 55645 MW; B8FE0943DC6A9F03 CRC64;
MKRKMMLINP HKPGRHGEES ITVIVQMPLN LAYVAALTPG DWEFDVIDEN LELAIDDNDE
ITFKPVDLVC ITSVTYQSPR AYKIAAACRK KGMTVIMGGI HASVMPEEAA NYVDSVFVGE
AEEVWPQVIK DFEEGKLKKV YQGGLPPLSL MKKVHPNREL MKKKYNYKFS SIVTTKGCPN
YCDFCSVPTF QGRKFRERPY EDVLDEMAAT DYKGLMLAED NFYGHGKKSN ERARDLFKGM
VERGIHKDWL GFTALNISQD PETLGYMAKS GNFGMLIGIE STNEAVLQKM NKRVNLKLGT
DSYFDCIQKI HDAGLVVWGS VVFGADGDDK DSFKRMTDFI LENNIDILTF GINCPFPKTQ
LYARLDSEKR IFRKNYPEDW QYYDTAHVVH RLVDMTLEDF IEGMQYVYDH VYAGDNLRTR
FRNSIKTTKN PRNSMFAFRV GSDWKQVFEQ VLQNLKELYD SGDYYKDCYK SSAAPVLKPI
MEAVTT
//
