ID A0A0D0QBV3_9RHOB Unreviewed; 453 AA.
AC A0A0D0QBV3;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Alpha-galactosidase {ECO:0000313|EMBL:KIQ69757.1};
DE EC=3.2.1.22 {ECO:0000313|EMBL:KIQ69757.1};
GN ORFNames=Wenmar_01327 {ECO:0000313|EMBL:KIQ69757.1};
OS Wenxinia marina DSM 24838.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Wenxinia.
OX NCBI_TaxID=1123501 {ECO:0000313|EMBL:KIQ69757.1, ECO:0000313|Proteomes:UP000035100};
RN [1] {ECO:0000313|EMBL:KIQ69757.1, ECO:0000313|Proteomes:UP000035100}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 24838 {ECO:0000313|EMBL:KIQ69757.1,
RC ECO:0000313|Proteomes:UP000035100};
RA Fiebig A., Goeker M., Klenk H.-P.P.;
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIQ69757.1}.
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DR EMBL; AONG01000008; KIQ69757.1; -; Genomic_DNA.
DR RefSeq; WP_018301312.1; NZ_KN848372.1.
DR AlphaFoldDB; A0A0D0QBV3; -.
DR STRING; 1123501.Wenmar_01327; -.
DR PATRIC; fig|1123501.6.peg.1411; -.
DR eggNOG; COG1486; Bacteria.
DR OrthoDB; 9767022at2; -.
DR Proteomes; UP000035100; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd05297; GH4_alpha_glucosidase_galactosidase; 1.
DR Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR InterPro; IPR019802; GlycHydrolase_4_CS.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR32092:SF6; ALPHA-GALACTOSIDASE; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS01324; GLYCOSYL_HYDROL_F4; 1.
PE 3: Inferred from homology;
KW Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000035100}.
FT DOMAIN 195..417
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT BINDING 148
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 170
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT BINDING 200
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT SITE 110
FT /note="Increases basicity of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ SEQUENCE 453 AA; 50783 MW; CD0A1EE04703BA70 CRC64;
MTIVTFIGAG STVFARNLAG DILQRPALRD ATIRLMDIDR KRLEESEIVV GKMARTLGGH
ATVETYTDRR RALDGADFVI CCLQVGGYEP CTVTDFEIPK KFGLRQTIAD TLGIGGIMRG
LRTVPVLWDI CADMREVCPD ALMLQYVNPM AINTWGIGAK FPDIRQVGLC HSVQGTAEEL
ARDLEIPIGE IRYHCAGINH MAFYLRFEHR QPDGSYRDLY PALRQGYREG RFPKPSSWNP
RCPNKVRYEM LMRLGHFVTE SSEHFAEYVP YFIKRDRPDL IERFGIPLDE YPKRCVEQIA
RWESEAQAYR EADEVVVKPS HEYASEIVNS VVTGAPSVVY GNIPNRGYIP QLPQGAAVEV
PVLVDANGLQ PTMVEGIPPQ LIALMRTNVN VQELTVAALV EEKVEHLYHA AMLDPHTAAE
LDLDQIWELT GELLAAHGEW VPEWARVGVK GAA
//
