ID A0A0D0Y7I8_9LACO Unreviewed; 304 AA.
AC A0A0D0Y7I8;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=L-lactate dehydrogenase {ECO:0000313|EMBL:KIS04233.1};
DE EC=1.1.1.27 {ECO:0000313|EMBL:KIS04233.1};
GN Name=ldh2 {ECO:0000313|EMBL:KIS04233.1};
GN ORFNames=WDC_0164 {ECO:0000313|EMBL:KIS04233.1};
OS Paucilactobacillus wasatchensis.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Paucilactobacillus.
OX NCBI_TaxID=1335616 {ECO:0000313|EMBL:KIS04233.1, ECO:0000313|Proteomes:UP000032279};
RN [1] {ECO:0000313|EMBL:KIS04233.1, ECO:0000313|Proteomes:UP000032279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WDC04 {ECO:0000313|EMBL:KIS04233.1,
RC ECO:0000313|Proteomes:UP000032279};
RA Oberg C.J., Culumber M., McMahon D.J., Broadbent J.R., Oberg T.S.,
RA Ortaki F.;
RT "Lactobacillus wasatchii sp. WDC04, a late gas producing bacteria isolated
RT from aged chedder cheese.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
CC {ECO:0000256|ARBA:ARBA00006054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIS04233.1}.
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DR EMBL; AWTT01000002; KIS04233.1; -; Genomic_DNA.
DR RefSeq; WP_044009905.1; NZ_AWTT01000002.1.
DR AlphaFoldDB; A0A0D0Y7I8; -.
DR STRING; 1335616.WDC_0164; -.
DR PATRIC; fig|1335616.4.peg.164; -.
DR OrthoDB; 9802969at2; -.
DR Proteomes; UP000032279; Unassembled WGS sequence.
DR GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR CDD; cd05291; HicDH_like; 1.
DR Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43128; L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+)); 1.
DR PANTHER; PTHR43128:SF34; L-LACTATE DEHYDROGENASE; 1.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW NAD {ECO:0000256|PIRSR:PIRSR000102-3};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003369,
KW ECO:0000313|EMBL:KIS04233.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000032279}.
FT DOMAIN 3..144
FT /note="Lactate/malate dehydrogenase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00056"
FT DOMAIN 147..301
FT /note="Lactate/malate dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02866"
FT ACT_SITE 177
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-1"
FT BINDING 8..13
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT BINDING 33
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
SQ SEQUENCE 304 AA; 33259 MW; 44CB4D5455E2F9E5 CRC64;
MRKFGIIGVG HVGATVAYTL VCKGIADELI LIDNNEGKAT AEQLDLQDAQ ARLDTRTIIK
TQDYSELEDT DVLFVTSGNI HALDNVSGNR WAEFEYTREI VKDIAPKIKA SGFDGVLIDT
MNPCDAITHY LQRETGLSRQ QCFGTGTFLD TARMQKVVSD VFDVDPKNVT GYVYGEHGES
QFSAWSTVQV NGLDILDLAH EQDLDLSELE DKARHGGWAV HSGKGYTSYA IATCAVKLSE
AVLSDAKLTC PCSSYSEKYG TYVGQPSIVG KNGIESVTEL PLPDDEEAKF KHSADTIKEK
FATL
//
