ID A0A0D0YW10_9LACO Unreviewed; 449 AA.
AC A0A0D0YW10;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE EC=3.1.11.6 {ECO:0000256|HAMAP-Rule:MF_00378};
DE AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE Short=Exonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
GN Name=xseA {ECO:0000256|HAMAP-Rule:MF_00378,
GN ECO:0000313|EMBL:KIS03459.1};
GN ORFNames=WDC_0974 {ECO:0000313|EMBL:KIS03459.1};
OS Paucilactobacillus wasatchensis.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Paucilactobacillus.
OX NCBI_TaxID=1335616 {ECO:0000313|EMBL:KIS03459.1, ECO:0000313|Proteomes:UP000032279};
RN [1] {ECO:0000313|EMBL:KIS03459.1, ECO:0000313|Proteomes:UP000032279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WDC04 {ECO:0000313|EMBL:KIS03459.1,
RC ECO:0000313|Proteomes:UP000032279};
RA Oberg C.J., Culumber M., McMahon D.J., Broadbent J.R., Oberg T.S.,
RA Ortaki F.;
RT "Lactobacillus wasatchii sp. WDC04, a late gas producing bacteria isolated
RT from aged chedder cheese.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC insoluble oligonucleotides, which are then degraded further into small
CC acid-soluble oligonucleotides. {ECO:0000256|HAMAP-Rule:MF_00378}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355};
CC -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00378}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355}.
CC -!- SIMILARITY: Belongs to the XseA family. {ECO:0000256|HAMAP-
CC Rule:MF_00378, ECO:0000256|RuleBase:RU004355}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIS03459.1}.
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DR EMBL; AWTT01000019; KIS03459.1; -; Genomic_DNA.
DR RefSeq; WP_044010722.1; NZ_AWTT01000019.1.
DR AlphaFoldDB; A0A0D0YW10; -.
DR STRING; 1335616.WDC_0974; -.
DR PATRIC; fig|1335616.4.peg.977; -.
DR OrthoDB; 9802795at2; -.
DR Proteomes; UP000032279; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04489; ExoVII_LU_OBF; 1.
DR HAMAP; MF_00378; Exonuc_7_L; 1.
DR InterPro; IPR003753; Exonuc_VII_L.
DR InterPro; IPR020579; Exonuc_VII_lsu_C.
DR InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR NCBIfam; TIGR00237; xseA; 1.
DR PANTHER; PTHR30008; EXODEOXYRIBONUCLEASE 7 LARGE SUBUNIT; 1.
DR PANTHER; PTHR30008:SF0; EXODEOXYRIBONUCLEASE 7 LARGE SUBUNIT; 1.
DR Pfam; PF02601; Exonuc_VII_L; 1.
DR Pfam; PF13742; tRNA_anti_2; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00378};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_00378};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00378};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00378};
KW Reference proteome {ECO:0000313|Proteomes:UP000032279}.
FT DOMAIN 7..102
FT /note="OB-fold nucleic acid binding"
FT /evidence="ECO:0000259|Pfam:PF13742"
FT DOMAIN 128..438
FT /note="Exonuclease VII large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02601"
FT COILED 272..300
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 449 AA; 50506 MW; A4BB609B106F67C1 CRC64;
MDNDKYLTVS ALTKYIKRKF DVDPYLGRIY LTGEISNFRP RPNAHQYFSL KDDHAKISAI
MFKSAFTKIK FQPEEGMKVL VTGRIGLYEA SGSYQIYVDS MQPDGVGALY QAYEQLKKKL
AQEGLFDAPK QLLPQFPKKI AVVTSPSGAV IRDIITTTRR RFPIAQLVLF PAVVQGDSAA
KDITRQIQRV NESGTFDTLI IGRGGGSIED LWPFNEEVVA RAIAASQVPV ISSVGHETDT
TIADLVADVR AATPTAAAEL AVPVLTDELL KITQQRQRLN NAMKNKIRLA QQNLTKLEHS
YIFTQPKRLY EGYLQNVDQV SNRLKQQINV TLQQWQQQVK LAASGLSMYA MNQRIVTATN
QSNELQQRLE QSGIRLIHDH RVVLQNTIAS LDFLSPLKIM SRGYTYVTYD NQVVKSVTEL
TTGSQVRLHF QDGQVQAEIN QINKEKNND
//
