ID A0A0D1A6Q9_9LACO Unreviewed; 581 AA.
AC A0A0D1A6Q9;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE SubName: Full=Pyruvate oxidase {ECO:0000313|EMBL:KIS03402.1};
DE EC=1.2.3.3 {ECO:0000313|EMBL:KIS03402.1};
GN Name=poxB {ECO:0000313|EMBL:KIS03402.1};
GN ORFNames=WDC_0981 {ECO:0000313|EMBL:KIS03402.1};
OS Paucilactobacillus wasatchensis.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Paucilactobacillus.
OX NCBI_TaxID=1335616 {ECO:0000313|EMBL:KIS03402.1, ECO:0000313|Proteomes:UP000032279};
RN [1] {ECO:0000313|EMBL:KIS03402.1, ECO:0000313|Proteomes:UP000032279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WDC04 {ECO:0000313|EMBL:KIS03402.1,
RC ECO:0000313|Proteomes:UP000032279};
RA Oberg C.J., Culumber M., McMahon D.J., Broadbent J.R., Oberg T.S.,
RA Ortaki F.;
RT "Lactobacillus wasatchii sp. WDC04, a late gas producing bacteria isolated
RT from aged chedder cheese.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the TPP enzyme family.
CC {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KIS03402.1}.
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DR EMBL; AWTT01000020; KIS03402.1; -; Genomic_DNA.
DR RefSeq; WP_044010729.1; NZ_AWTT01000020.1.
DR AlphaFoldDB; A0A0D1A6Q9; -.
DR STRING; 1335616.WDC_0981; -.
DR PATRIC; fig|1335616.4.peg.984; -.
DR OrthoDB; 4494979at2; -.
DR Proteomes; UP000032279; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0047112; F:pyruvate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR CDD; cd02014; TPP_POX; 1.
DR CDD; cd07039; TPP_PYR_POX; 1.
DR Gene3D; 1.10.10.940; -; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR047211; POXB-like.
DR InterPro; IPR014092; Pyruvate_oxidase.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR InterPro; IPR000399; TPP-bd_CS.
DR InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR InterPro; IPR047212; TPP_POXB-like.
DR InterPro; IPR047210; TPP_PYR_POXB-like.
DR NCBIfam; TIGR02720; pyruv_oxi_spxB; 1.
DR PANTHER; PTHR42981; PYRUVATE DEHYDROGENASE [UBIQUINONE]; 1.
DR PANTHER; PTHR42981:SF2; PYRUVATE DEHYDROGENASE [UBIQUINONE]; 1.
DR Pfam; PF02775; TPP_enzyme_C; 1.
DR Pfam; PF00205; TPP_enzyme_M; 1.
DR Pfam; PF02776; TPP_enzyme_N; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR PROSITE; PS00187; TPP_ENZYMES; 1.
PE 3: Inferred from homology;
KW Oxidoreductase {ECO:0000313|EMBL:KIS03402.1};
KW Pyruvate {ECO:0000313|EMBL:KIS03402.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000032279};
KW Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132}.
FT DOMAIN 6..120
FT /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF02776"
FT DOMAIN 196..325
FT /note="Thiamine pyrophosphate enzyme central"
FT /evidence="ECO:0000259|Pfam:PF00205"
FT DOMAIN 385..531
FT /note="Thiamine pyrophosphate enzyme TPP-binding"
FT /evidence="ECO:0000259|Pfam:PF02775"
SQ SEQUENCE 581 AA; 63261 MW; 0BBA2AA98C8046E6 CRC64;
MTNKINAADA MIKVLEDWQI DHIFGLPGGS FDSTMNALYN RQNSIKYVQV RHEETGALAA
AGEAKFTGKI GVTFGSAGPG AVHLLNGLYD AKHDGVPVLA LVGQVGTDFM NTEYFQELNE
NPIFADVAVY NRTVMTAEQL PAVVDQAIRQ AYEHSGVAVV TIPKDLGWAE IDDDYISTAG
NYAVTENYPL PDKVAVKQAA EILQNAKRPV IYFGLGAKKA GPELKRLSDR LKIPLMSSAL
AKGVIADDDP AYMGSAGRVA TKPGVETARA ADAILFIGSN FPFAAYFFSP TAKFIQVDVK
PTNIGKRHHV DVGILADAKT TLTALTNQVE EVGKRAFYEA ALANKREWRD WLAKFVDEDK
QPLNVDSVFE QINRIATDDA IFALDVGNVT IDGVRLLQMK PTQKMATSGW YATMGFALPA
AIGAQFSYPN RQVFSISGDG GYTMVMHDIL TQVKYQQPII NVVLTNQSLG FIEAEQDDTK
QPHSGVDLID ANFGAAATDM GATGYEVHTL SELHQAFDDA KSTNGPVVID VKIANDRPLP
VEQLVLTTDD QHSQTDVDQF IKQYHAEGLT PFSEFLAQYK A
//
