ID A0A0D1YJ47_ANEMI Unreviewed; 419 AA.
AC A0A0D1YJ47;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Small RNA 2'-O-methyltransferase {ECO:0000256|ARBA:ARBA00021330};
DE EC=2.1.1.386 {ECO:0000256|ARBA:ARBA00035025};
GN ORFNames=AF333_14500 {ECO:0000313|EMBL:KON96505.1}, SAMN04487909_10221
GN {ECO:0000313|EMBL:SDI18053.1};
OS Aneurinibacillus migulanus (Bacillus migulanus).
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
OC Aneurinibacillus group; Aneurinibacillus.
OX NCBI_TaxID=47500 {ECO:0000313|EMBL:KON96505.1, ECO:0000313|Proteomes:UP000037269};
RN [1] {ECO:0000313|EMBL:KON96505.1, ECO:0000313|Proteomes:UP000037269}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2895 {ECO:0000313|EMBL:KON96505.1,
RC ECO:0000313|Proteomes:UP000037269};
RA Liu B., Wang J., Zhu Y., Liu G., Chen Q., Chen Z., Lan J., Che J., Ge C.,
RA Shi H., Pan Z., Liu X.;
RT "Fjat-14205 dsm 2895.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:SDI18053.1, ECO:0000313|Proteomes:UP000182836}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2895 {ECO:0000313|EMBL:SDI18053.1,
RC ECO:0000313|Proteomes:UP000182836};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H(+) +
CC S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide;
CC Xref=Rhea:RHEA:37887, Rhea:RHEA-COMP:10415, Rhea:RHEA-COMP:10416,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74896, ChEBI:CHEBI:74898; EC=2.1.1.386;
CC Evidence={ECO:0000256|ARBA:ARBA00034992};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family.
CC {ECO:0000256|ARBA:ARBA00009026}.
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DR EMBL; LGUG01000004; KON96505.1; -; Genomic_DNA.
DR EMBL; FNED01000002; SDI18053.1; -; Genomic_DNA.
DR RefSeq; WP_043064187.1; NZ_LGUG01000004.1.
DR AlphaFoldDB; A0A0D1YJ47; -.
DR STRING; 47500.AF333_14500; -.
DR PATRIC; fig|47500.8.peg.1702; -.
DR OrthoDB; 626362at2; -.
DR Proteomes; UP000037269; Unassembled WGS sequence.
DR Proteomes; UP000182836; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProtKB-KW.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR026610; Hen1.
DR InterPro; IPR025714; Methyltranfer_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR21404; HEN1; 1.
DR PANTHER; PTHR21404:SF3; SMALL RNA 2'-O-METHYLTRANSFERASE; 1.
DR Pfam; PF13847; Methyltransf_31; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:KON96505.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000037269};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KON96505.1}.
FT DOMAIN 249..369
FT /note="Methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF13847"
SQ SEQUENCE 419 AA; 49481 MW; 19F78D1BC49DB7E9 CRC64;
MAIIQIKSSN PDFSFIIKKN PASGMQLRSI RKGIAYGWYH GTDTYNIYFK DADTDISYKQ
HRDETFEYLN VSRYNTPLLP LNAIAEFLSA TFKNKNEKDK DGYENSFFVN MIHIKYPRYI
DFFNKHFTDF HIEIEQHTHK SYKMTIKSNR SIYKLVNFAN VLFLFLAMLG KEYLGVSDSL
VEKYLKSMNI IDAPYYIRYL FARNVLINKE IFYKFKKELE QTDKYAINFV FGNTAIQRKQ
FIEKGISFDK SILDIGCGEG FYALPFSGKI EDNFYYAIDI NEELIREVDI KAKKRNIENL
ITFPSLHTYL ESYSGEVVDV LLTEVIEHMD VPEAELLVKS VYENVKFDKF IITTPNYDFN
QYYALNEFRH SDHKWEMNKN EFEKWIHDMF KETNYTLQFL NIGDSVDGVY TTQGVLITT
//
