ID A0A0D2BHQ9_9EURO Unreviewed; 328 AA.
AC A0A0D2BHQ9;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=nitrilase {ECO:0000256|ARBA:ARBA00039045};
DE EC=3.5.5.1 {ECO:0000256|ARBA:ARBA00039045};
GN ORFNames=PV06_10853 {ECO:0000313|EMBL:KIW36952.1};
OS Exophiala oligosperma.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala.
OX NCBI_TaxID=215243 {ECO:0000313|EMBL:KIW36952.1, ECO:0000313|Proteomes:UP000053342};
RN [1] {ECO:0000313|EMBL:KIW36952.1, ECO:0000313|Proteomes:UP000053342}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 72588 {ECO:0000313|EMBL:KIW36952.1,
RC ECO:0000313|Proteomes:UP000053342};
RG The Broad Institute Genomics Platform;
RA Cuomo C., de Hoog S., Gorbushina A., Stielow B., Teixiera M.,
RA Abouelleil A., Chapman S.B., Priest M., Young S.K., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Exophiala oligosperma CBS72588.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a nitrile + 2 H2O = a carboxylate + NH4(+);
CC Xref=Rhea:RHEA:21724, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29067; EC=3.5.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00036406};
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC Nitrilase family. {ECO:0000256|ARBA:ARBA00008129}.
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DR EMBL; KN847347; KIW36952.1; -; Genomic_DNA.
DR RefSeq; XP_016257168.1; XM_016412443.1.
DR AlphaFoldDB; A0A0D2BHQ9; -.
DR STRING; 215243.A0A0D2BHQ9; -.
DR GeneID; 27362927; -.
DR VEuPathDB; FungiDB:PV06_10853; -.
DR HOGENOM; CLU_030130_6_0_1; -.
DR OrthoDB; 2785533at2759; -.
DR Proteomes; UP000053342; Unassembled WGS sequence.
DR GO; GO:0000257; F:nitrilase activity; IEA:UniProt.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR CDD; cd07564; nitrilases_CHs; 1.
DR Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR000132; Nitrilase/CN_hydratase_CS.
DR InterPro; IPR044149; Nitrilases_CHs.
DR PANTHER; PTHR46044:SF1; CN HYDROLASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR46044; NITRILASE; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
DR PROSITE; PS00920; NITRIL_CHT_1; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000053342}.
FT DOMAIN 9..286
FT /note="CN hydrolase"
FT /evidence="ECO:0000259|PROSITE:PS50263"
FT ACT_SITE 49
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10139"
SQ SEQUENCE 328 AA; 36740 MW; ECA789F97A54AC04 CRC64;
MSAPYNKTVK IAAIQAEPVW NDLQGGVEKA ISLIKEAGKN GANVLGFPEV FIPGYPWAIW
SQSVNDCAAF MDEYYRNSLV KESPEMDRIR DAVREAGIFI VLGYSERDNG SLYIAQSFID
PTGTIIHHRR KIKPTHVERA YYGDGQSESL TTTAQTPFGK VSGLNCWEHS QLLLRYYTYW
QNTDIHVASW PLIWDMPSDG SPWQWHITPD ASNRFSQVMA MEGGCFVMTA TQILTEKNKK
KCLVDNYPYA RAPGGGFSMI FGPDGKELVK PLDPGEEGIL YADVDLSVRA LAKHNLDTVG
HYSRPDLLSL RVTKHGVKHP GTQVHFLE
//
