ID A0A0D2BXJ0_9EURO Unreviewed; 596 AA.
AC A0A0D2BXJ0;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|ARBA:ARBA00017788, ECO:0000256|RuleBase:RU368004};
DE EC=2.1.1.211 {ECO:0000256|ARBA:ARBA00012795, ECO:0000256|RuleBase:RU368004};
GN ORFNames=PV05_05738 {ECO:0000313|EMBL:KIW57146.1};
OS Exophiala xenobiotica.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala.
OX NCBI_TaxID=348802 {ECO:0000313|EMBL:KIW57146.1, ECO:0000313|Proteomes:UP000054342};
RN [1] {ECO:0000313|EMBL:KIW57146.1, ECO:0000313|Proteomes:UP000054342}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 118157 {ECO:0000313|EMBL:KIW57146.1,
RC ECO:0000313|Proteomes:UP000054342};
RG The Broad Institute Genomics Platform;
RA Cuomo C., de Hoog S., Gorbushina A., Stielow B., Teixiera M.,
RA Abouelleil A., Chapman S.B., Priest M., Young S.K., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Exophiala xenobiotica CBS118157.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00000660,
CC ECO:0000256|RuleBase:RU368004};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368004}.
CC -!- SIMILARITY: Belongs to the TRM44 family.
CC {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
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DR EMBL; KN847319; KIW57146.1; -; Genomic_DNA.
DR RefSeq; XP_013317730.1; XM_013462276.1.
DR AlphaFoldDB; A0A0D2BXJ0; -.
DR STRING; 348802.A0A0D2BXJ0; -.
DR GeneID; 25327646; -.
DR HOGENOM; CLU_018580_0_0_1; -.
DR OrthoDB; 1353816at2759; -.
DR Proteomes; UP000054342; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW Methyltransferase {ECO:0000256|RuleBase:RU368004};
KW Reference proteome {ECO:0000313|Proteomes:UP000054342};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW tRNA processing {ECO:0000256|RuleBase:RU368004}.
SQ SEQUENCE 596 AA; 66427 MW; ED198F51E57DB294 CRC64;
MAITRFCSAG VPWETAEEYQ KECPCLTEDK FMQVTNLLLE NPNLNSTHLF RADILQDSAQ
ILRTVAEKEQ QCQYINGGQD AENGTITDSP DTLEKAPQLD GATLTRKVLR RLIPRKPQLD
RPLNQWCYVY NLSKNPNIDK GHVVVYVPDV ESEADMPWYH PPVQSLAYFY KRTGSETTLS
IHYLPFTRST DPLPSRLHRT FISLLQRFLR LAKNPAPAQS LNGSHAQSDS ITATTQSMTP
SALKDTILPQ HVVQDTYSRL KQRYAQDLIS RWAESTEPSK HVFEDLSIAA FVIELWKQMY
TPDTFPGFVD IACGNGVLSY VLIQEGYQGR GFDARRRKSW AVLGMNDHLD EMICVPQPFL
DAASEDEGLQ ELMMRAQIYD GLFKPGTFII SNHADELTPW TPVLAALSCP EHPLPFLAIP
CCSHALSGSK HRYSAKDIII HNRGVAASSA KLNDQEEQPA SGDLKSLRAA KVKAASHTDD
KSMYACLTRK VVALAEEIGF DVELTLMRIP STRNIGIVGN RKGASTSRDA SNLEQQLETL
SINSGDTTKK IHNLIDRECA VSGRIQEAAA TWIERAEKLQ KGSGRGKVNL GGKQQA
//
