ID A0A0D2H3Q1_9EURO Unreviewed; 517 AA.
AC A0A0D2H3Q1;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Nitrate reductase [NADPH] {ECO:0000256|ARBA:ARBA00015499};
DE EC=1.7.1.3 {ECO:0000256|ARBA:ARBA00012673};
GN ORFNames=Z518_05880 {ECO:0000313|EMBL:KIX05008.1};
OS Rhinocladiella mackenziei CBS 650.93.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae;
OC Rhinocladiella.
OX NCBI_TaxID=1442369 {ECO:0000313|EMBL:KIX05008.1, ECO:0000313|Proteomes:UP000053617};
RN [1] {ECO:0000313|EMBL:KIX05008.1, ECO:0000313|Proteomes:UP000053617}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 650.93 {ECO:0000313|EMBL:KIX05008.1,
RC ECO:0000313|Proteomes:UP000053617};
RG The Broad Institute Genomics Platform;
RA Cuomo C., de Hoog S., Gorbushina A., Stielow B., Teixiera M.,
RA Abouelleil A., Chapman S.B., Priest M., Young S.K., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Rhinocladiella mackenzie CBS 650.93.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NADP(+) + nitrite = H(+) + NADPH + nitrate;
CC Xref=Rhea:RHEA:19061, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.7.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00000195};
CC -!- COFACTOR:
CC Name=Mo-molybdopterin; Xref=ChEBI:CHEBI:71302;
CC Evidence={ECO:0000256|ARBA:ARBA00001924};
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DR EMBL; KN847478; KIX05008.1; -; Genomic_DNA.
DR RefSeq; XP_013272144.1; XM_013416690.1.
DR AlphaFoldDB; A0A0D2H3Q1; -.
DR STRING; 1442369.A0A0D2H3Q1; -.
DR GeneID; 25293951; -.
DR VEuPathDB; FungiDB:Z518_05880; -.
DR HOGENOM; CLU_003827_5_1_1; -.
DR OrthoDB; 1239at2759; -.
DR Proteomes; UP000053617; Unassembled WGS sequence.
DR GO; GO:0030151; F:molybdenum ion binding; IEA:InterPro.
DR GO; GO:0050464; F:nitrate reductase (NADPH) activity; IEA:UniProtKB-EC.
DR Gene3D; 2.60.40.650; -; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR Gene3D; 3.90.420.10; Oxidoreductase, molybdopterin-binding domain; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR005066; MoCF_OxRdtse_dimer.
DR InterPro; IPR008335; Mopterin_OxRdtase_euk.
DR InterPro; IPR000572; OxRdtase_Mopterin-bd_dom.
DR InterPro; IPR036374; OxRdtase_Mopterin-bd_sf.
DR PANTHER; PTHR19372:SF7; SULFITE OXIDASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR19372; SULFITE REDUCTASE; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF03404; Mo-co_dimer; 1.
DR Pfam; PF00174; Oxidored_molyb; 1.
DR PRINTS; PR00407; EUMOPTERIN.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF56524; Oxidoreductase molybdopterin-binding domain; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000053617}.
FT DOMAIN 41..119
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
SQ SEQUENCE 517 AA; 57826 MW; 4F114C1D708DB0ED CRC64;
MPRIEPGEGS LSQPAATTII FASAISPLAV AHAETPATET QRLIRLKEVK QHGKTAERKW
VIKGNRVFDI TDWIAAHPGG PVILRAVGGS IDKYWDIFSI HKKQEVYDIL EGYFIGEIDP
RDLVDGHVAA NDIEDPFVSD PTRDSRLLEH TAKPCNAETP TSCLGSFITP NETFYVRNHF
WVPDSDPNSH ILTVELPDGT EKEYSCHDIE SKFEPFQRTV TLQCSGNRRK HMSEGARPTS
GLPWDAGAIS NAEWTGVRLR DVLEDAGFPI NQPNTENARH VHFIGAEAYG TSIPIDKALD
PNGDVMLAYK MAGKPLPRDH GAPLRVLVPG YTAARSVKWL EKIILAEEES QSQWQQRDYK
CFGPNQTAND VDWSSAPAIQ ETPVQSAITS IRSISNASRA DRKELTTYGM QEDIIKVEGY
AYSGGGRRVV RVDVSADGGK SWHQATILPD NAQGHKTWSW KRWEWMVPKR MAGRTFVVKA
VDDSYNVQPE SYEAQWNFRG VLTNAWHKVD YPNKDQP
//
