ID A0A0D2IQV4_9EURO Unreviewed; 1084 AA.
AC A0A0D2IQV4;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=tRNA wybutosine-synthesizing protein 4 {ECO:0000256|ARBA:ARBA00018045};
DE EC=2.1.1.290 {ECO:0000256|ARBA:ARBA00012779};
DE EC=2.3.1.231 {ECO:0000256|ARBA:ARBA00012155};
DE AltName: Full=Leucine carboxyl methyltransferase 2 {ECO:0000256|ARBA:ARBA00030231};
DE AltName: Full=tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase {ECO:0000256|ARBA:ARBA00030847};
DE AltName: Full=tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase {ECO:0000256|ARBA:ARBA00029750};
GN ORFNames=Z518_03010 {ECO:0000313|EMBL:KIX08354.1};
OS Rhinocladiella mackenziei CBS 650.93.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae;
OC Rhinocladiella.
OX NCBI_TaxID=1442369 {ECO:0000313|EMBL:KIX08354.1, ECO:0000313|Proteomes:UP000053617};
RN [1] {ECO:0000313|EMBL:KIX08354.1, ECO:0000313|Proteomes:UP000053617}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 650.93 {ECO:0000313|EMBL:KIX08354.1,
RC ECO:0000313|Proteomes:UP000053617};
RG The Broad Institute Genomics Platform;
RA Cuomo C., de Hoog S., Gorbushina A., Stielow B., Teixiera M.,
RA Abouelleil A., Chapman S.B., Priest M., Young S.K., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Rhinocladiella mackenzie CBS 650.93.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase
CC that acts as a component of the wybutosine biosynthesis pathway.
CC Wybutosine is a hyper modified guanosine with a tricyclic base found at
CC the 3'-position adjacent to the anticodon of eukaryotic phenylalanine
CC tRNA. May methylate the carboxyl group of leucine residues to form
CC alpha-leucine ester residues. {ECO:0000256|ARBA:ARBA00025588}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in
CC tRNA(Phe) + CO2 + S-adenosyl-L-methionine = 2 H(+) + S-adenosyl-L-
CC homocysteine + wybutosine(37) in tRNA(Phe); Xref=Rhea:RHEA:37119,
CC Rhea:RHEA-COMP:11844, Rhea:RHEA-COMP:11847, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73544, ChEBI:CHEBI:74275; EC=2.3.1.231;
CC Evidence={ECO:0000256|ARBA:ARBA00000401};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-
CC adenosyl-L-methionine = 7-[(3S)-(3-amino-3-
CC methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36903, Rhea:RHEA-COMP:10379, Rhea:RHEA-
CC COMP:11844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73543,
CC ChEBI:CHEBI:74275; EC=2.1.1.290;
CC Evidence={ECO:0000256|ARBA:ARBA00001806};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family.
CC {ECO:0000256|ARBA:ARBA00010703}.
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DR EMBL; KN847476; KIX08354.1; -; Genomic_DNA.
DR RefSeq; XP_013275490.1; XM_013420036.1.
DR AlphaFoldDB; A0A0D2IQV4; -.
DR STRING; 1442369.A0A0D2IQV4; -.
DR GeneID; 25291081; -.
DR VEuPathDB; FungiDB:Z518_03010; -.
DR HOGENOM; CLU_002761_1_0_1; -.
DR OrthoDB; 9938at2759; -.
DR UniPathway; UPA00375; -.
DR Proteomes; UP000053617; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.650; Cupin; 1.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR041667; Cupin_8.
DR InterPro; IPR003347; JmjC_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR007213; Ppm1/Ppm2/Tcmp.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR46529; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4; 1.
DR PANTHER; PTHR46529:SF1; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4; 1.
DR Pfam; PF13621; Cupin_8; 1.
DR Pfam; PF04072; LCM; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51184; JMJC; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000053617};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 841..1007
FT /note="JmjC"
FT /evidence="ECO:0000259|PROSITE:PS51184"
SQ SEQUENCE 1084 AA; 121798 MW; DB90DF5C5D89C090 CRC64;
MTSPITPRSM GSDDEIPMER NAGLIMGTNN SSIVSKRSVE RLYLPQPHFY RYFVRKPQRR
SPTINRGYWL RMRAIDWVVR QFLERPSDQK KVIINLGCGY DPIPFQWMSQ DRELCSNTRF
IDVDFEELMI SKREIILNTP KMRDMLSLIG DLPLEKGIIL DTDEYVALGC DLRNLRRLEH
LLKSVVDIEQ CLVLFIAEVS ITYMATEDSD ALIAWTTSLA PGKSPDRPDN PFTAAMLNHF
SKLGTPLRSV LTYPGNHTQT LRFQGAGFSI VDIQSLWELW ADPRFLSPSQ RMSLDEVEPF
DEWEEFALFA SHYCLIIAQT GPEPLIPEQP RSRRASIDSF ASDLSTRTVS PYRGKTQWFA
YKYSANPDRE GRTHHASAFF IPGQDAIGVH GGVGLKNRLS STSVYASADS KVSAPTLPPE
DVGARCCHTI TSLANGWNVL VGGRGSPSAP MKDCWLQTES GWERIQDLPE PRFRHRAVAV
VLPYNEYGVV VFGGKTSATK VALDCLLWDR VNGWQKLRSL RSDPMPRFGA SFVRLGFNHG
LLFGGMRHDG VICQGLWRWR LIIRDNAVAG ISFKTSTALD TSAGIWPWLA RLGASYSVIR
NELLVIGGVA KHGCIPKDYE ILSILGSFSA FGEFEKEMEL RVACVVPKKD PDVPRPFLIG
HSTHRSGKET TLLVGGGATC FSFGAYWNPG CWLLYDREAG ISSHWALIKP APVKALPPPV
PKDDSPTPQP MSIERIRLCD EKEFTDILRE GIPKVFASLD IGPCISEWTP CKLLSKIPPS
KSIVVHSAAT RTMNFQRKDF VYTAMPFHEF INQLENNQDA HMYLRSISST NPTQLPANFN
TDWPELSSDF QLPTPLRFIK QTQHSSPLRI SANVNMWLHY DVMANVLFQV RGRRKLILFP
SEDIKYLSFP AGATTSTLDI LEAAEHPEKE EDRLKPIPNT HPHITILRPG EALFIPPLWA
HSGTPMPPQP PSNVDAPSPD NSRINISLNV FFRTIPATMY PAGRDVYGNR DLAAYEDARR
DLDKIVRRFT AISVKQKVNG DNSSASTTTQ GDVPVDIHES IPKPIAKAYL ERLADELKEK
AERL
//
