ID   A0A0D2JNN9_9CHLO        Unreviewed;       769 AA.
AC   A0A0D2JNN9;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   22-FEB-2023, entry version 31.
DE   SubName: Full=tRNA uridine 5-carboxymethylaminomethylmodification enzyme mnmG {ECO:0000313|EMBL:KIZ00768.1};
GN   ORFNames=MNEG_7195 {ECO:0000313|EMBL:KIZ00768.1};
OS   Monoraphidium neglectum.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Sphaeropleales; Selenastraceae; Monoraphidium.
OX   NCBI_TaxID=145388 {ECO:0000313|EMBL:KIZ00768.1, ECO:0000313|Proteomes:UP000054498};
RN   [1] {ECO:0000313|EMBL:KIZ00768.1, ECO:0000313|Proteomes:UP000054498}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAG 48.87 {ECO:0000313|EMBL:KIZ00768.1,
RC   ECO:0000313|Proteomes:UP000054498};
RX   PubMed=24373495; DOI=10.1186/1471-2164-14-926;
RA   Bogen C., Al-Dilaimi A., Albersmeier A., Wichmann J., Grundmann M.,
RA   Rupp O., Lauersen K.J., Blifernez-Klassen O., Kalinowski J., Goesmann A.,
RA   Mussgnug J.H., Kruse O.;
RT   "Reconstruction of the lipid metabolism for the microalga Monoraphidium
RT   neglectum from its genome sequence reveals characteristics suitable for
RT   biofuel production.";
RL   BMC Genomics 14:926-926(2013).
CC   -!- FUNCTION: Involved in the 5-carboxymethylaminomethyl modification
CC       (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs.
CC       {ECO:0000256|ARBA:ARBA00002739}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- SIMILARITY: Belongs to the MnmG family.
CC       {ECO:0000256|ARBA:ARBA00007653}.
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DR   EMBL; KK101471; KIZ00768.1; -; Genomic_DNA.
DR   RefSeq; XP_013899787.1; XM_014044333.1.
DR   AlphaFoldDB; A0A0D2JNN9; -.
DR   STRING; 145388.A0A0D2JNN9; -.
DR   GeneID; 25740071; -.
DR   KEGG; mng:MNEG_7195; -.
DR   OrthoDB; 5486689at2759; -.
DR   Proteomes; UP000054498; Unassembled WGS sequence.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:InterPro.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 3.
DR   Gene3D; 1.10.150.570; GidA associated domain, C-terminal subdomain; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR047001; MnmG_C_subdom.
DR   InterPro; IPR044920; MnmG_C_subdom_sf.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; GLUCOSE INHIBITED DIVISION PROTEIN A; 1.
DR   PANTHER; PTHR11806:SF0; PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL; 1.
DR   Pfam; PF01134; GIDA; 3.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SMART; SM01228; GIDA_assoc_3; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Reference proteome {ECO:0000313|Proteomes:UP000054498}.
FT   DOMAIN          660..731
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme C-terminal subdomain"
FT                   /evidence="ECO:0000259|SMART:SM01228"
FT   REGION          105..152
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          285..311
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          732..769
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        105..150
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   769 AA;  77345 MW;  8872C46E7BDA6C70 CRC64;
     MGCEAAAAAA RRGARVLLVT PSPTTSIGAM SCNPSIGGVG KGHLVREVDA MGGLMARVAD
     AAGIQFHLLN SAKGPAVRGP RAQMDRAAYR AEMQAAIYAI ANGGSSGSSG SSGSSGSSGS
     SGSSGSSSSS SSSGGGSSGS SGSSSGSVEG GPGTAAAAAF GGGGRGSLVV VDGVVDDLLL
     EHAAAPGGRA AARGVRLASG ERLLARSVVL TTGTFLRGTI HIGSKSFPAG RLKDSAAAAA
     DAGSAAAPAA ASHAATDAAD DAAAHGGTAL ARTVAAAGFR LGRLKTGTPP RLDARTVDFS
     GMSPQPTDDA PVPLSFANLS TPGWRPRLPQ RCNWEARTTA ATEALVARCV AAGRGWLPPE
     GVAGPSSRTT RRSATTATNP QVWLEPEGVD SHVLYPNGLS CSLEPEDQLE MLRTIPGLEH
     AAMLAPAYAV EYDYVVPTEL THTLEARRVA GLYLAGQING TTGYEEAAAQ GLLAGANAAR
     PDAPLILSRA DAYLGVMVDD LVGRGTSEPY RMMSSRAEYR LRLRPDNADI RLGPIAEAAG
     LLSSSQAEAL AERRDEVAAW RGALERAATP PAVWRWRGLA IEGGGHKRVS AAQLLAWPGV
     ELPAVVAAAL AATSDGCAVE ATQPDALAGS SDGGAAELAA LAALPGGALR SAAATAAADV
     LYAPYEARQE EEISRLRADE ALELPHDLDY GAVQGLSSEE REALAKARPA TLAAAARVAG
     VGHGGLTALL QAARPRAVRP RGRGLEPAGA AGGNEPQLSQ LPPKVAGKP
//