ID A0A0D2PZ96_GOSRA Unreviewed; 531 AA.
AC A0A0D2PZ96;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=B456_001G150900 {ECO:0000313|EMBL:KJB09581.1}, Gorai_021273
GN {ECO:0000313|EMBL:MBA0579004.1};
OS Gossypium raimondii (New World cotton).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=29730 {ECO:0000313|EMBL:KJB09581.1, ECO:0000313|Proteomes:UP000032304};
RN [1] {ECO:0000313|EMBL:KJB09581.1, ECO:0000313|Proteomes:UP000032304}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23257886; DOI=10.1038/nature11798;
RA Paterson A.H., Wendel J.F., Gundlach H., Guo H., Jenkins J., Jin D.,
RA Llewellyn D., Showmaker K.C., Shu S., Udall J., Yoo M.J., Byers R.,
RA Chen W., Doron-Faigenboim A., Duke M.V., Gong L., Grimwood J., Grover C.,
RA Grupp K., Hu G., Lee T.H., Li J., Lin L., Liu T., Marler B.S., Page J.T.,
RA Roberts A.W., Romanel E., Sanders W.S., Szadkowski E., Tan X., Tang H.,
RA Xu C., Wang J., Wang Z., Zhang D., Zhang L., Ashrafi H., Bedon F.,
RA Bowers J.E., Brubaker C.L., Chee P.W., Das S., Gingle A.R., Haigler C.H.,
RA Harker D., Hoffmann L.V., Hovav R., Jones D.C., Lemke C., Mansoor S.,
RA ur Rahman M., Rainville L.N., Rambani A., Reddy U.K., Rong J.K.,
RA Saranga Y., Scheffler B.E., Scheffler J.A., Stelly D.M., Triplett B.A.,
RA Van Deynze A., Vaslin M.F., Waghmare V.N., Walford S.A., Wright R.J.,
RA Zaki E.A., Zhang T., Dennis E.S., Mayer K.F., Peterson D.G., Rokhsar D.S.,
RA Wang X., Schmutz J.;
RT "Repeated polyploidization of Gossypium genomes and the evolution of
RT spinnable cotton fibres.";
RL Nature 492:423-427(2012).
RN [2] {ECO:0000313|EMBL:MBA0579004.1, ECO:0000313|Proteomes:UP000593578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=8 {ECO:0000313|EMBL:MBA0579004.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:MBA0579004.1};
RX PubMed=30476109;
RA Grover C.E., Arick M.A. 2nd, Thrash A., Conover J.L., Sanders W.S.,
RA Peterson D.G., Frelichowski J.E., Scheffler J.A., Scheffler B.E.,
RA Wendel J.F.;
RT "Insights into the Evolution of the New World Diploid Cottons (Gossypium,
RT Subgenus Houzingenia) Based on Genome Sequencing.";
RL Genome Biol. Evol. 11:53-71(2019).
RN [3] {ECO:0000313|EMBL:MBA0579004.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=8 {ECO:0000313|EMBL:MBA0579004.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:MBA0579004.1};
RA Grover C.E., Arick M.A. II, Thrash A., Conover J.L., Sanders W.S.,
RA Peterson D.G., Scheffler J.A., Scheffler B.E., Wendel J.F.;
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
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DR EMBL; CM001740; KJB09581.1; -; Genomic_DNA.
DR EMBL; JABEZZ010000001; MBA0579004.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0D2PZ96; -.
DR STRING; 29730.A0A0D2PZ96; -.
DR EnsemblPlants; KJB09581; KJB09581; B456_001G150900.
DR Gramene; KJB09581; KJB09581; B456_001G150900.
DR eggNOG; ENOG502QPKZ; Eukaryota.
DR OMA; DSFLNMT; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000032304; Chromosome 1.
DR Proteomes; UP000593578; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF73; 3-KETOACYL-COA SYNTHASE 1; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000032304};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 97..118
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 116..404
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 422..502
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 260
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 339
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 423
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 427
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 456
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 460
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 531 AA; 59924 MW; 400A2E1A5185E48A CRC64;
MDKDSIEMDK ERLTAEMAFK DSSSAVIKIR RRLPDFLQSV KLKYVKLGYG YSCNPATILM
VALVLPLLYT ILVHFIDLKM DRISELWTNQ SLRLESIDAA TTLAASLFMF FLFALYYAKR
SRPVYLVDFA CFKPEDDRKM SVDSFLKMTE DSGAFAEDTL QFQRRISTRS GLGDETYFPR
GITSTPPNLC MEEARSEAEA VMFGALDSLF EKTGVNPKDI GILVVNCSLF NPTPSLSAMI
VKHYKLRTDI KSYNLGGMGC SAGLISVELA KNLLQANPNT YAVVVSTENI TLNWYFGNDR
SMLLCNCIFR MGGAAVLLSN KAGDKSRSKY QLVHLVRTHK GADDKHYNCV YQREDEKGTV
GVSLARELMA VAGDALKTNI TTLGPLVLPW TEQFMFFVTL VRKKIFKAKV KPYIPDFKLA
FEHFCIHAGG RAVLDELQKN LQLTDWHMEP SRMTLHRFGN TSSSSLWYEL AYTEAKGRVS
SGDRVWQIAF GSGFKCNSAV WRALRRSPMN ELRGNPWKDE IDKQPVKVPI A
//
