ID A0A0D2T8V6_GOSRA Unreviewed; 911 AA.
AC A0A0D2T8V6;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 03-MAY-2023, entry version 26.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN ORFNames=B456_011G160300 {ECO:0000313|EMBL:KJB72128.1};
OS Gossypium raimondii (New World cotton).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=29730 {ECO:0000313|EMBL:KJB72128.1, ECO:0000313|Proteomes:UP000032304};
RN [1] {ECO:0000313|EMBL:KJB72128.1, ECO:0000313|Proteomes:UP000032304}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23257886; DOI=10.1038/nature11798;
RA Paterson A.H., Wendel J.F., Gundlach H., Guo H., Jenkins J., Jin D.,
RA Llewellyn D., Showmaker K.C., Shu S., Udall J., Yoo M.J., Byers R.,
RA Chen W., Doron-Faigenboim A., Duke M.V., Gong L., Grimwood J., Grover C.,
RA Grupp K., Hu G., Lee T.H., Li J., Lin L., Liu T., Marler B.S., Page J.T.,
RA Roberts A.W., Romanel E., Sanders W.S., Szadkowski E., Tan X., Tang H.,
RA Xu C., Wang J., Wang Z., Zhang D., Zhang L., Ashrafi H., Bedon F.,
RA Bowers J.E., Brubaker C.L., Chee P.W., Das S., Gingle A.R., Haigler C.H.,
RA Harker D., Hoffmann L.V., Hovav R., Jones D.C., Lemke C., Mansoor S.,
RA ur Rahman M., Rainville L.N., Rambani A., Reddy U.K., Rong J.K.,
RA Saranga Y., Scheffler B.E., Scheffler J.A., Stelly D.M., Triplett B.A.,
RA Van Deynze A., Vaslin M.F., Waghmare V.N., Walford S.A., Wright R.J.,
RA Zaki E.A., Zhang T., Dennis E.S., Mayer K.F., Peterson D.G., Rokhsar D.S.,
RA Wang X., Schmutz J.;
RT "Repeated polyploidization of Gossypium genomes and the evolution of
RT spinnable cotton fibres.";
RL Nature 492:423-427(2012).
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; CM001750; KJB72128.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0D2T8V6; -.
DR EnsemblPlants; KJB72128; KJB72128; B456_011G160300.
DR Gramene; KJB72128; KJB72128; B456_011G160300.
DR OMA; HDYHESS; -.
DR Proteomes; UP000032304; Chromosome 11.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000032304}.
FT DOMAIN 44..363
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 493..854
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
FT REGION 27..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 911 AA; 101496 MW; AE7A3EF43E45A7B1 CRC64;
MEAIQMVRLG IRLWSYVREE ASHGRKAPID PFTRESCKPS ASQGVPLGGM GSGSISRGFR
GEFRQWQIIP GTCNISPVMA NQFSIFISRD GGNKKYASVL APGQHEGLGK TSDEGISSWG
WNLNGQHSTY HALFPRAWTI YDGEPDPDLK VSCRQISPFV PHDYRDSSLP TAVFVYTLVN
TGKERAKVSL LFTWANSIGG ISHLSGDHVN EPFIGEDGVS GVLLHHKTAK GNPPVTFAVA
ASETQNVNVT VLPCFGLTEG SFITARKIWG KMMQDGQFDR ENFNIGPSMP SSPGETLCAA
VSASAWVEPH GKCTIAFALA WSSPKIKFSK GSSYHRRYTK FYGTSERAAV DLVHDALTNY
KHWEEEIEKW QSPILEDIRL PEWYKFTLFN ELYFLVAGGT VWIDSSSPSA DVKSDQDPPI
KVESKNIKVA EAEMNCRHST GFEHTSTSGC NSSTGVGLKQ NGSSTIPRKK RSCKHFSHHL
KTEDQLDGNE DVGRFLYLEG VEYIMWCTYD VHFYASFALL ELFPKIELNI QRDFAKAVLS
EDGRKVKFLA EGNYGIRKVR GAVPHDLGTH DPWNEMNAYN IHDTSKWKDL NSKFVLQVYR
DFAATGDMAF GIEVWPAVRT AMEYMEQFDR DDDGLIENDG FPDQTYDAWT VHGVSAYCGC
LWLAALQAAA AMAQRLGDKF FAETCKTKFF SAKSAFEKKL WNGSYFNYDS GSSSNSKSIQ
ADQLAGQWYT SSSGLGPIFD EFKTRSSLQK IFDFNVMKVK GGRMGAVNGM HPNGKVDESC
MQSREVWTGV TYAVAANMLL AGMEEEAFTT AEGIFIAGWS EEGFGYWFQT PEGWTTNGHY
RSLIYMRPLA IWSMQWVLSP PKAIIEAPKI NMMDRIVISP ATFSLSLPET RVRKIANKTA
CFGNSSLQCT C
//
