ID A0A0D2UUN6_GOSRA Unreviewed; 969 AA.
AC A0A0D2UUN6;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN ORFNames=B456_011G160300 {ECO:0000313|EMBL:KJB72126.1};
OS Gossypium raimondii (New World cotton).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=29730 {ECO:0000313|EMBL:KJB72126.1, ECO:0000313|Proteomes:UP000032304};
RN [1] {ECO:0000313|EMBL:KJB72126.1, ECO:0000313|Proteomes:UP000032304}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23257886; DOI=10.1038/nature11798;
RA Paterson A.H., Wendel J.F., Gundlach H., Guo H., Jenkins J., Jin D.,
RA Llewellyn D., Showmaker K.C., Shu S., Udall J., Yoo M.J., Byers R.,
RA Chen W., Doron-Faigenboim A., Duke M.V., Gong L., Grimwood J., Grover C.,
RA Grupp K., Hu G., Lee T.H., Li J., Lin L., Liu T., Marler B.S., Page J.T.,
RA Roberts A.W., Romanel E., Sanders W.S., Szadkowski E., Tan X., Tang H.,
RA Xu C., Wang J., Wang Z., Zhang D., Zhang L., Ashrafi H., Bedon F.,
RA Bowers J.E., Brubaker C.L., Chee P.W., Das S., Gingle A.R., Haigler C.H.,
RA Harker D., Hoffmann L.V., Hovav R., Jones D.C., Lemke C., Mansoor S.,
RA ur Rahman M., Rainville L.N., Rambani A., Reddy U.K., Rong J.K.,
RA Saranga Y., Scheffler B.E., Scheffler J.A., Stelly D.M., Triplett B.A.,
RA Van Deynze A., Vaslin M.F., Waghmare V.N., Walford S.A., Wright R.J.,
RA Zaki E.A., Zhang T., Dennis E.S., Mayer K.F., Peterson D.G., Rokhsar D.S.,
RA Wang X., Schmutz J.;
RT "Repeated polyploidization of Gossypium genomes and the evolution of
RT spinnable cotton fibres.";
RL Nature 492:423-427(2012).
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; CM001750; KJB72126.1; -; Genomic_DNA.
DR RefSeq; XP_012456249.1; XM_012600795.1.
DR AlphaFoldDB; A0A0D2UUN6; -.
DR STRING; 29730.A0A0D2UUN6; -.
DR EnsemblPlants; KJB72126; KJB72126; B456_011G160300.
DR GeneID; 105777488; -.
DR Gramene; KJB72126; KJB72126; B456_011G160300.
DR eggNOG; KOG2119; Eukaryota.
DR OrthoDB; 997839at2759; -.
DR Proteomes; UP000032304; Chromosome 11.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000032304}.
FT DOMAIN 102..421
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 551..912
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
FT REGION 85..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 969 AA; 108285 MW; 5CCB40BA38638DF2 CRC64;
MVTGNLFHSK KNSWPPEEYV SRNTLQLFNF DSAAPPKQAW RRRLNSHANI LKEFSVTFME
AIQMVRLGIR LWSYVREEAS HGRKAPIDPF TRESCKPSAS QGVPLGGMGS GSISRGFRGE
FRQWQIIPGT CNISPVMANQ FSIFISRDGG NKKYASVLAP GQHEGLGKTS DEGISSWGWN
LNGQHSTYHA LFPRAWTIYD GEPDPDLKVS CRQISPFVPH DYRDSSLPTA VFVYTLVNTG
KERAKVSLLF TWANSIGGIS HLSGDHVNEP FIGEDGVSGV LLHHKTAKGN PPVTFAVAAS
ETQNVNVTVL PCFGLTEGSF ITARKIWGKM MQDGQFDREN FNIGPSMPSS PGETLCAAVS
ASAWVEPHGK CTIAFALAWS SPKIKFSKGS SYHRRYTKFY GTSERAAVDL VHDALTNYKH
WEEEIEKWQS PILEDIRLPE WYKFTLFNEL YFLVAGGTVW IDSSSPSADV KSDQDPPIKV
ESKNIKVAEA EMNCRHSTGF EHTSTSGCNS STGVGLKQNG SSTIPRKKRS CKHFSHHLKT
EDQLDGNEDV GRFLYLEGVE YIMWCTYDVH FYASFALLEL FPKIELNIQR DFAKAVLSED
GRKVKFLAEG NYGIRKVRGA VPHDLGTHDP WNEMNAYNIH DTSKWKDLNS KFVLQVYRDF
AATGDMAFGI EVWPAVRTAM EYMEQFDRDD DGLIENDGFP DQTYDAWTVH GVSAYCGCLW
LAALQAAAAM AQRLGDKFFA ETCKTKFFSA KSAFEKKLWN GSYFNYDSGS SSNSKSIQAD
QLAGQWYTSS SGLGPIFDEF KTRSSLQKIF DFNVMKVKGG RMGAVNGMHP NGKVDESCMQ
SREVWTGVTY AVAANMLLAG MEEEAFTTAE GIFIAGWSEE GFGYWFQTPE GWTTNGHYRS
LIYMRPLAIW SMQWVLSPPK AIIEAPKINM MDRIVISPAT FSLSLPETRV RKIANKTACF
GNSSLQCTC
//
