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Database: UniProt
Entry: A0A0D2V6J2_GOSRA
LinkDB: A0A0D2V6J2_GOSRA
Original site: A0A0D2V6J2_GOSRA 
ID   A0A0D2V6J2_GOSRA        Unreviewed;       837 AA.
AC   A0A0D2V6J2;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   31-JUL-2019, entry version 29.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   ORFNames=B456_012G175100 {ECO:0000313|EMBL:KJB78051.1};
OS   Gossypium raimondii (New World cotton).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae;
OC   Gossypium.
OX   NCBI_TaxID=29730 {ECO:0000313|EMBL:KJB78051.1, ECO:0000313|Proteomes:UP000032304};
RN   [1] {ECO:0000313|EMBL:KJB78051.1, ECO:0000313|Proteomes:UP000032304}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23257886; DOI=10.1038/nature11798;
RA   Paterson A.H., Wendel J.F., Gundlach H., Guo H., Jenkins J., Jin D.,
RA   Llewellyn D., Showmaker K.C., Shu S., Udall J., Yoo M.J., Byers R.,
RA   Chen W., Doron-Faigenboim A., Duke M.V., Gong L., Grimwood J.,
RA   Grover C., Grupp K., Hu G., Lee T.H., Li J., Lin L., Liu T.,
RA   Marler B.S., Page J.T., Roberts A.W., Romanel E., Sanders W.S.,
RA   Szadkowski E., Tan X., Tang H., Xu C., Wang J., Wang Z., Zhang D.,
RA   Zhang L., Ashrafi H., Bedon F., Bowers J.E., Brubaker C.L., Chee P.W.,
RA   Das S., Gingle A.R., Haigler C.H., Harker D., Hoffmann L.V., Hovav R.,
RA   Jones D.C., Lemke C., Mansoor S., ur Rahman M., Rainville L.N.,
RA   Rambani A., Reddy U.K., Rong J.K., Saranga Y., Scheffler B.E.,
RA   Scheffler J.A., Stelly D.M., Triplett B.A., Van Deynze A.,
RA   Vaslin M.F., Waghmare V.N., Walford S.A., Wright R.J., Zaki E.A.,
RA   Zhang T., Dennis E.S., Mayer K.F., Peterson D.G., Rokhsar D.S.,
RA   Wang X., Schmutz J.;
RT   "Repeated polyploidization of Gossypium genomes and the evolution of
RT   spinnable cotton fibres.";
RL   Nature 492:423-427(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
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DR   EMBL; CM001751; KJB78048.1; -; Genomic_DNA.
DR   EMBL; CM001751; KJB78051.1; -; Genomic_DNA.
DR   RefSeq; XP_012458531.1; XM_012603077.1.
DR   RefSeq; XP_012458532.1; XM_012603078.1.
DR   STRING; 29730.Gorai.012G175100.1; -.
DR   EnsemblPlants; KJB78048; KJB78048; B456_012G175100.
DR   EnsemblPlants; KJB78051; KJB78051; B456_012G175100.
DR   GeneID; 105779332; -.
DR   Gramene; KJB78048; KJB78048; B456_012G175100.
DR   Gramene; KJB78051; KJB78051; B456_012G175100.
DR   KEGG; gra:105779332; -.
DR   KO; K01427; -.
DR   OMA; GFDSHIH; -.
DR   OrthoDB; 183108at2759; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000032304; Chromosome 12.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR040881; Urease_linker.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   Pfam; PF18473; Urease_linker; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000032304};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000032304}.
FT   DOMAIN      399    837       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    590    590       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       404    404       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       406    406       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       487    487       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       487    487       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       516    516       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       542    542       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       630    630       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     489    489       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     487    487       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   837 AA;  90136 MW;  7AABEE1C888E5AB4 CRC64;
     MKLTPKEVEK LGLHNAGYLA QKRLARGLRL NYTEAVALIA TQILEFVRDG NWSVAELMDI
     GPRLLGWRQV LPAVPHLLDS VQVEGTFPDG TKLITVHNVV NCENGDLHLA LYGSFLPVPS
     PDKFLEMEDK MVPGDLIFKD GIISLNLGRK AVRLNVTNRG DRPVQVGSHY HFIEVNPYMV
     FDRRRAYGMR LNIRAGTATR FEPGESKPVV LVSIGGNKVI RGGNGIVDGP VDYNNIETVM
     ETIRIEGYGN TEDADASEGL TGEDSDFTTT VSPEAYANMY GPTTGDKIRL GDTNLYAEIE
     QDFAIYGDEC VFGGGKVIRD GMGQSCGHLP DKSLDIVITN AVIIDYTGIF KADIGVKNGC
     IVGLGKAGNP DTMDGVSENM TIGINTEVIA GEGLIVTAGA IDCHVHFICP QLVHEAISSG
     ITTLVGGGTG PAEGTRATTC TPAPSQMKMM LQSTDDFPLN FGFTGKGNGS KPEEIHEIIK
     AGAMGLKLHE DWGTTPAAID SCLAVAELYD IQVNIHTDTL NESGFVEQTI NAFKGRTIHT
     YHSEGAGGGH APDIIKVCGV KNVLPSSTNP TRPYTSNTID EHLDMLMVCH HLSKDIPEDV
     AFAESRIRAE TIAAEDILHD TGAISIISSD SQAMGRIGEV ICRTWQTAHK MKSQRGPLGP
     ADSDNDNLRI KRYIAKYTIN AAIANGIAEF VGSVEVGKLA DLVLWKPSFF GAKPEMVIKG
     GEVTWANMGD ANASIPTPEP VLSRPMFGAF GKAASANSIA FVSKAALDRG VKESYGLQKR
     VEAVGNTRNL TKLDMKLNDA LPNIEVDPET YIVKADGEVL TCDAATTVPL SRNYFLF
//
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