ID A0A0D2ZQL0_BRAOL Unreviewed; 848 AA.
AC A0A0D2ZQL0;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo00689s050.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo00689s050.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TO1000 {ECO:0000313|EnsemblPlants:Bo00689s050.1};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo00689s050.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR AlphaFoldDB; A0A0D2ZQL0; -.
DR STRING; 109376.A0A0D2ZQL0; -.
DR EnsemblPlants; Bo00689s050.1; Bo00689s050.1; Bo00689s050.
DR Gramene; Bo00689s050.1; Bo00689s050.1; Bo00689s050.
DR eggNOG; KOG0987; Eukaryota.
DR HOGENOM; CLU_001324_12_1_1; -.
DR OMA; DETFHMH; -.
DR Proteomes; UP000032141; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR025476; Helitron_helicase-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR45786; DNA BINDING PROTEIN-LIKE; 1.
DR PANTHER; PTHR45786:SF66; REPLICATION FACTOR-A CARBOXY-TERMINAL DOMAIN PROTEIN; 1.
DR Pfam; PF14214; Helitron_like_N; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT DOMAIN 328..450
FT /note="Helitron helicase-like"
FT /evidence="ECO:0000259|Pfam:PF14214"
FT DOMAIN 689..734
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
FT REGION 24..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 451..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..467
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 848 AA; 97065 MW; 4F542120D27541DD CRC64;
MASTPILTSK ARSAIDKENI LVHSTTKPNV TQGNPIPPAK RLRRVAPANS TTSSKGKNRY
IDEGDATYKC DHCGAIMWYG ERINRKRYAR KPKFSMCCGH GQVQLPLLKE FPDILKILLT
EDDEMSRYFR ENIRVINIVF SFTSLGGKVD RSVQKGIGPQ MFQLQGENYH LMGSLKPPEG
EPKKYKKPAE KTRKEELMKK VIETLMAMLD ECNPYIHQFR SARDRFDTNP DETFHMHIIS
SREKDGRTYD TPTASEVASL IPGDFNLEMD KRDIVLQEKQ TGWLRRISEI HSAYLALHYP
LIFTYGEDGF RLGIKKRATD ATAKLKRQTI SMRQCLRSDS YDSIQQAENA GKTDMHEQGS
RLLLPASFTR GPRYMKNMYL DAMAICRRIG FPDLFITFTC NPKWPEVTRY LQPRKLAAED
RPEILCRLFK IKLKSLMTDL TDKQLLGKTV SDNGASKEQT APNAEPNEES KNEIKDFFNC
RKTNHIFKDD DTYEEVTSRK LIDNTMFMGW FEFCKPIPDI INENVLIMDE LSYDRQELKA
AHDRDFPKLT DEQRKIYDLI TDDVFSKKRG VFFVYGFSGT GKTFLWRLLS AAIRYRGEIC
LNVASSGIAS LLLPGGRTTH SRFGIPINPY EFSTCNLIPG TDQANLVKAA SLIIWDEAPM
MSDEKFYLSS DSIDSSDRFS KKDQALTPDF QHKIKASGLP NHSLRLKIGC PVMLLRNIDP
IGGLMNGTRL QITEMYDFMI KAKVITWEKV GRIVLLPRLS ITPSDKKLEV GLFLPRHVFS
HGQLYVAISR VTSKKGLKIL RVDNEGKPQR QTMNVVFKEI FSNLCYHFAV VFRSCCTTSY
LILLPIHF
//
