GenomeNet

Database: UniProt
Entry: A0A0D2ZSD6_BRAOL
LinkDB: A0A0D2ZSD6_BRAOL
Original site: A0A0D2ZSD6_BRAOL 
ID   A0A0D2ZSD6_BRAOL        Unreviewed;       953 AA.
AC   A0A0D2ZSD6;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   28-MAR-2018, entry version 19.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   Name=106320478 {ECO:0000313|EnsemblPlants:Bo00935s020.1};
OS   Brassica oleracea var. oleracea.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae;
OC   Brassica.
OX   NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo00935s020.1, ECO:0000313|Proteomes:UP000032141};
RN   [1] {ECO:0000313|EnsemblPlants:Bo00935s020.1, ECO:0000313|Proteomes:UP000032141}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TO1000 {ECO:0000313|EnsemblPlants:Bo00935s020.1,
RC   ECO:0000313|Proteomes:UP000032141};
RX   PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA   Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA   Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F.,
RA   Belcram H., Links M.G., Just J., Clarke C., Bender T., Huebert T.,
RA   Mason A.S., Pires J.C., Barker G., Moore J., Walley P.G., Manoli S.,
RA   Batley J., Edwards D., Nelson M.N., Wang X., Paterson A.H., King G.,
RA   Bancroft I., Chalhoub B., Sharpe A.G.;
RT   "Transcriptome and methylome profiling reveals relics of genome
RT   dominance in the mesopolyploid Brassica oleracea.";
RL   Genome Biol. 15:R77-R77(2014).
RN   [2] {ECO:0000313|EnsemblPlants:Bo00935s020.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   RefSeq; XP_013614304.1; XM_013758850.1.
DR   EnsemblPlants; Bo00935s020.1; Bo00935s020.1; Bo00935s020.
DR   GeneID; 106320478; -.
DR   Gramene; Bo00935s020.1; Bo00935s020.1; Bo00935s020.
DR   KEGG; boe:106320478; -.
DR   KO; K17108; -.
DR   OMA; HDYQDSS; -.
DR   Proteomes; UP000032141; Unplaced.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:EnsemblPlants.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000032141};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT   DOMAIN       90    408       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      525    886       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   953 AA;  106214 MW;  4838BCDF625498D5 CRC64;
     MSEENFMDNG EDVKSSATKV DPAIPASLTW QRKIDSNAKA PREFALTAKE ILQMAPIGIR
     LWFLVREEAA KGRLAFIDPF SKHSVTSSHG VPLGGIGSGS IGRSFKGEFQ RWQLFPPKCE
     DEPVLANQFS AFVSRANGRK YSSVLCPRNP KLAKQESESG IGSWDWNMKG DKATYHALYP
     RSWTIYEGEP DPELRIVCRQ VSPFIPHNYK ESSFPVSVFT FTIHNLGDAT ADATLLFTWA
     NSVGGDSEFS GGHYNSKIMM NDGVKGVLLH HKTANGLPSL SYAVSAQETE GVSVSVCPFF
     TVSGKQNGIT AKDMWEIIKE HGSFDHLDAS EASMQSEHGS SIGAAVAASA TVLPGESRII
     TFSLAWDCPE VQFPSGKIYS RRYTKFYGTH GDAAAQIAYD AILGHSQWES WIEDWQRPIL
     EDKRLPAWYP ITLFNELYYL NSGGTLWTDG SSPLHSLAGV REKKFSLDKS QSGLKSIIDV
     PQQQNDTAVS VLEKMASTLE QLHASTASNS AFGTKLLGEG EENIGHFLYL EGIEYRMWNT
     YDVHFYASFA LVMLFPKLEL SIQRDFAAAV MLHDPTKVKT LSEGQWVQRK VLGAVPHDLG
     INDPWFEVNG YNLHNTDRWK DLNPKFVLQV YRDVVATGDK KFALAVWPSV YVAMAYMAQF
     DKDGDGMIEN EGFPDQTYDT WSASGVSAYC GGLWVAALQA ASALAREVGD KNSQDYFWSK
     FEKAKVVYEK KLWNGSYFNY DTSGSRYSSS IQADQLAGQW YARASGLMPI VDEDKARMAL
     EKVYNFNVMK IKDGKRGAVN GMHPDGKVDT ASMQSREIWS GVTYALSATM IQEGLVDKAF
     QTASGIYEAA WSETGLGYSF QTPEAWNTND QYRALTYMRP LAIWSMQWAL TRTSNNKQKQ
     FGLEPELEPE PSSIMKHDIG FSRVSRLLNL PNEASAKGTV QTLFEYACRR MMS
//
DBGET integrated database retrieval system