ID A0A0D3A9D8_BRAOL Unreviewed; 722 AA.
AC A0A0D3A9D8;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=pyruvate kinase {ECO:0000256|ARBA:ARBA00012142};
DE EC=2.7.1.40 {ECO:0000256|ARBA:ARBA00012142};
GN Name=106319996 {ECO:0000313|EnsemblPlants:Bo1g078680.1};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo1g078680.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo1g078680.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo1g078680.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- COFACTOR:
CC Name=K(+); Xref=ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00001958};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 5/5. {ECO:0000256|ARBA:ARBA00004997}.
CC -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC {ECO:0000256|ARBA:ARBA00008663}.
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DR RefSeq; XP_013613849.1; XM_013758395.1.
DR RefSeq; XP_013613895.1; XM_013758441.1.
DR AlphaFoldDB; A0A0D3A9D8; -.
DR STRING; 109376.A0A0D3A9D8; -.
DR EnsemblPlants; Bo1g078680.1; Bo1g078680.1; Bo1g078680.
DR GeneID; 106319996; -.
DR Gramene; Bo1g078680.1; Bo1g078680.1; Bo1g078680.
DR KEGG; boe:106319996; -.
DR eggNOG; KOG2323; Eukaryota.
DR HOGENOM; CLU_015439_6_0_1; -.
DR OMA; VWATQVL; -.
DR OrthoDB; 454163at2759; -.
DR UniPathway; UPA00109; UER00188.
DR Proteomes; UP000032141; Chromosome C1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR GO; GO:0004743; F:pyruvate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 2.
DR InterPro; IPR001697; Pyr_Knase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR InterPro; IPR011037; Pyrv_Knase-like_insert_dom_sf.
DR InterPro; IPR015793; Pyrv_Knase_brl.
DR PANTHER; PTHR11817:SF76; PLASTIDIAL PYRUVATE KINASE 4, CHLOROPLASTIC; 1.
DR PANTHER; PTHR11817; PYRUVATE KINASE; 1.
DR Pfam; PF00224; PK; 2.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF50800; PK beta-barrel domain-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyruvate {ECO:0000256|ARBA:ARBA00023317};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 245..331
FT /note="Pyruvate kinase barrel"
FT /evidence="ECO:0000259|Pfam:PF00224"
FT DOMAIN 483..691
FT /note="Pyruvate kinase barrel"
FT /evidence="ECO:0000259|Pfam:PF00224"
SQ SEQUENCE 722 AA; 79495 MW; B63AA76858BA19A0 CRC64;
MTSYMSSAPS VTNLWTTPNN VIQLADMVST ESYHLLSGKK TLIFSLRPTK SFPLSLTQMK
IRVPRTLAFA SAKGKGKAES GVEAIVGDNK SSTERNWSFD FPESKAEFCL VDSEAYQNLS
SVLEKLNALR SHLLAAKKWN ASRLQSCDSK YLECATNLIH YMALRSLDTE QLNNYLASLG
LSSLDNDNNL SVLSNLDATI NLLMKSPMES WKQQKGNKII EKNDKGRVLS SYKESLLLGK
LREGKKTHIM VTVGKEATES ETFITDILKA GASVIRINCA HGDPTLWGEI IKRVRRTSQM
LEMPCRILMD LAGPKLRTGT LKPGPCVMKV SPKKDAYGNV ASPAIVWLSV TETEPPAHLS
PDATIFVQDQ EFLAGLQIGD SVRLYDARGK KKKLRISKEF DVFSSTGFVA ECFDTAYVEP
GTELCVKGNK GRRLFGEVVD VPPKESFVRL RVGDLLVITR EGSFDEPSVT VPGAHRLTCP
SGYLFDSVKP GETIGFDDGK IWGTIKGASP AEVIVSITHA GPKGTKLGSE KSINIPQSDI
RFKGLTSKDI KDLEYVASHA DMVGISFIRD VQDITVLRQE LKKRKLNDQL GVVLKIETES
GFENLPLILL EAMKCLNPLG VMIARGDLAV ECGWERLANL QEEIMAICKV ARMPVILATQ
VLESLVKSGV PTRAEITDAA NGRRASCVML NKGKHIVEAV SMLDTILHTK LIYKKQESEN
LH
//
