ID A0A0D3B330_BRAOL Unreviewed; 754 AA.
AC A0A0D3B330;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo3g019600.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo3g019600.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo3g019600.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; A0A0D3B330; -.
DR STRING; 109376.A0A0D3B330; -.
DR EnsemblPlants; Bo3g019600.1; Bo3g019600.1; Bo3g019600.
DR Gramene; Bo3g019600.1; Bo3g019600.1; Bo3g019600.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR OMA; THFEQWN; -.
DR Proteomes; UP000032141; Chromosome C3.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-KW.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF146; BETA-GALACTOSIDASE 4; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Apoplast {ECO:0000256|ARBA:ARBA00022523};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT DOMAIN 68..371
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 395..449
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 648..732
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 754 AA; 84777 MW; 14439C866466911B CRC64;
PLTLYILLQN NYQTQKPHLN RIFFYLSGKS KEMGSNFRDK ALIFLAFLCY SSLICSVKAT
VSYDRKAVII NGQRRILLSG SIHYPRSTPE MWSGLIQKAK EGGLDVIETY VFWNGHEPSP
GNYYFGDRYD LVKFIKLVHQ AGLYVNLRIG PYVCAEWNFG GFPVWLKFVP GMAFRTDNEP
FKAAMKKFTE KIVWMMKAEK LFQTQGGPII LAQIENEYGP VEWEIGAPGK AYTKWVAQMA
LGLSTGVPWI MCKQEDAPSP IIDTCNGYYC ENFKPNSNNK PKMWTENWTG WYTEFGGAVP
YRPVEDIAYS VARFIQNGGS FVNYYMYHGG TNFDRTAGEF MASSYDYDAP LDEYGLTREP
KYSHLKALHK VIKLSEPALV SADATVTSLG AKQEAHVFWS KSSCAAFLSN NDANSAARVM
FRGFPYDLPP WSVSILPDCK TEYYNTAKVN APRVHRNMVP TGTRFSWESF NEATPSANER
DTFARNGLVE QISMTWDKSD YFWYLTDITI GAGERFLQTG DYPLLTIWSA GHALHVFVNG
QLAGSAYGGL SHPKLTFSQK IKLHAGVNKL ALLSVAVGLP NVGQHFETWN KGVLGPVTLK
GVNSGTWDMS KWKWSYKIGV KGEAMSLHTD SSSVSWNQGS YVVKKQPLTW YKSTFSAPGG
NEPLALDMNT MGKGHVWING RNIGRHWPAY KAQGNCGRCN YAGTFDAKKC LSNCGEASQR
WYHVPRSWLK SQNLIVVFEE WGGDPSGISL VKRT
//
