ID A0A0D3C686_BRAOL Unreviewed; 584 AA.
AC A0A0D3C686;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=thioglucosidase {ECO:0000256|ARBA:ARBA00012250};
DE EC=3.2.1.147 {ECO:0000256|ARBA:ARBA00012250};
DE AltName: Full=Sinigrinase {ECO:0000256|ARBA:ARBA00032643};
DE AltName: Full=Thioglucosidase {ECO:0000256|ARBA:ARBA00032797};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo4g194990.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo4g194990.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo4g194990.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- FUNCTION: Degradation of glucosinolates (glucose residue linked by a
CC thioglucoside bound to an amino acid derivative) to glucose, sulfate
CC and any of the products: thiocyanates, isothiocyanates, nitriles,
CC epithionitriles or oxazolidine-2-thiones.
CC {ECO:0000256|ARBA:ARBA00003014}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a thioglucoside + H2O = a sugar + a thiol.; EC=3.2.1.147;
CC Evidence={ECO:0000256|ARBA:ARBA00034026};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838, ECO:0000256|RuleBase:RU003690}.
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DR AlphaFoldDB; A0A0D3C686; -.
DR STRING; 109376.A0A0D3C686; -.
DR EnsemblPlants; Bo4g194990.1; Bo4g194990.1; Bo4g194990.
DR Gramene; Bo4g194990.1; Bo4g194990.1; Bo4g194990.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR OMA; VRTIIYH; -.
DR Proteomes; UP000032141; Chromosome C4.
DR GO; GO:0102799; F:glucosinolate glucohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0019137; F:thioglucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353:SF253; BETA-GLUCOSIDASE 28; 1.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..584
FT /note="thioglucosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002259078"
SQ SEQUENCE 584 AA; 67923 MW; 157A0B4850F1C6B6 CRC64;
MNVKFFILLL TISWLFKKIT SLPPESRVLD RRSFPDNFVF GTAASAFQYE GATSEGGKSP
SIWDYFSHTF PERTNMQNGD VAVDFYHRYK DDIKLMKELD MDAFRFSISW ARLIPSGKVK
DGVNKEGVKF YNALIDELIA NGIQPSMSLY HWDHPQTLED EYGGFLSPQI IEDFRDFSRV
CFEEFGDRVK MWTTINEPYI ITVAGYDTGN KAVGRCSKWV NSQCKAGDSA TEPYIASHHL
LLAHAAAVQE FRKCNKTSQD GQIGIVLSPL WFEPYDSTSL EDNEAVKRAL VIELDWHLDP
VIHGDYPEIL KKQLRNRLPS FTAEQSKMLK NSSDFIGVNY YTGRYIAHLP YVDPARPRFK
TDQQLEWRGI TNHSNHQIGP EEDRGILHSV PEGLRKVLNY IKDKYSNPTV YIKENGINDY
DDGTKSREDI LNDTFRIKYH KDHLQQLHKA IMEDGCDVKG YYAWSLLDNF EWEHGYNTRF
GLYYVDYDNN LERYPKDSVN WFKRFLNRLD VKSEIKKEEV WDVSRNNKTL DDDAKGFEAS
VRTIIYHTTN SSRREEEEEK DRFTLEVPYN RLNLSLGSYT SFGL
//
