ID A0A0D3CPM4_BRAOL Unreviewed; 296 AA.
AC A0A0D3CPM4;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=16S rRNA (uracil(1498)-N(3))-methyltransferase {ECO:0000256|ARBA:ARBA00012328};
DE EC=2.1.1.193 {ECO:0000256|ARBA:ARBA00012328};
GN Name=106296547 {ECO:0000313|EnsemblPlants:Bo6g019800.1};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo6g019800.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo6g019800.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo6g019800.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00025699}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC Evidence={ECO:0000256|ARBA:ARBA00000792};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000256|ARBA:ARBA00005528}.
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DR RefSeq; XP_013588150.1; XM_013732696.1.
DR AlphaFoldDB; A0A0D3CPM4; -.
DR STRING; 109376.A0A0D3CPM4; -.
DR EnsemblPlants; Bo6g019800.1; Bo6g019800.1; Bo6g019800.
DR GeneID; 106296547; -.
DR Gramene; Bo6g019800.1; Bo6g019800.1; Bo6g019800.
DR KEGG; boe:106296547; -.
DR eggNOG; ENOG502QPPV; Eukaryota.
DR HOGENOM; CLU_067442_0_0_1; -.
DR OMA; RCITQWK; -.
DR OrthoDB; 324904at2759; -.
DR Proteomes; UP000032141; Chromosome C6.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IEA:InterPro.
DR CDD; cd18084; RsmE-like; 1.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; RsmE.
DR InterPro; IPR046886; RsmE_MTase_dom.
DR InterPro; IPR046887; RsmE_PUA-like.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR NCBIfam; TIGR00046; RsmE family RNA methyltransferase; 1.
DR PANTHER; PTHR30027:SF3; 16S RRNA (URACIL(1498)-N(3))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR30027; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE E; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR Pfam; PF20260; PUA_4; 1.
DR SUPFAM; SSF75217; alpha/beta knot; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 72..116
FT /note="Ribosomal RNA small subunit methyltransferase E PUA-
FT like"
FT /evidence="ECO:0000259|Pfam:PF20260"
FT DOMAIN 132..286
FT /note="Ribosomal RNA small subunit methyltransferase E
FT methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04452"
FT REGION 24..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 296 AA; 32160 MW; AA7C40638ECA9AD5 CRC64;
MRALAAARPL FSDLPSRLRS WLPPSSSSFK TFSASSSSPP SDSDLRNQSR GGLPRFFSDD
LPSRKGGMVR VQGSEFWHMA KVLRLKAEDR VELFNGKGGL VEGCIQNIDK TGVDFVAQED
QKVILPQGIQ WQVFAAFGTL KGGRADWLIE KCTELGASSV TPLLTERSSI ISDNRVDRLE
RVSFAAAKQC QRLHQMVVSP AVKFNTLLDH ISKSKLCLVA TAEATPLLNA VQSSAKESSG
LLIVGPEGDF TKKEVEMMLE AGGTAVGLGP HRLRVETATI ALLATLVMWS DSQETD
//
