GenomeNet

Database: UniProt
Entry: A0A0D3CS03_BRAOL
LinkDB: A0A0D3CS03_BRAOL
Original site: A0A0D3CS03_BRAOL 
ID   A0A0D3CS03_BRAOL        Unreviewed;       950 AA.
AC   A0A0D3CS03;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   28-MAR-2018, entry version 14.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   Name=106300556 {ECO:0000313|EnsemblPlants:Bo6g047890.1};
OS   Brassica oleracea var. oleracea.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae;
OC   Brassica.
OX   NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo6g047890.1, ECO:0000313|Proteomes:UP000032141};
RN   [1] {ECO:0000313|EnsemblPlants:Bo6g047890.1, ECO:0000313|Proteomes:UP000032141}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX   PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA   Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA   Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F.,
RA   Belcram H., Links M.G., Just J., Clarke C., Bender T., Huebert T.,
RA   Mason A.S., Pires J.C., Barker G., Moore J., Walley P.G., Manoli S.,
RA   Batley J., Edwards D., Nelson M.N., Wang X., Paterson A.H., King G.,
RA   Bancroft I., Chalhoub B., Sharpe A.G.;
RT   "Transcriptome and methylome profiling reveals relics of genome
RT   dominance in the mesopolyploid Brassica oleracea.";
RL   Genome Biol. 15:R77-R77(2014).
RN   [2] {ECO:0000313|EnsemblPlants:Bo6g047890.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (MAR-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   RefSeq; XP_013592176.1; XM_013736722.1.
DR   EnsemblPlants; Bo6g047890.1; Bo6g047890.1; Bo6g047890.
DR   GeneID; 106300556; -.
DR   Gramene; Bo6g047890.1; Bo6g047890.1; Bo6g047890.
DR   KEGG; boe:106300556; -.
DR   KO; K17108; -.
DR   OMA; KGGMCGA; -.
DR   Proteomes; UP000032141; Chromosome C6.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005773; C:vacuole; IEA:EnsemblPlants.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000032141};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT   DOMAIN       85    411       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      523    883       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   950 AA;  106152 MW;  18278D92FC14A698 CRC64;
     MQNGHTETED QNQMAIDDKL PPISWERKLN SQTKTPSEFK LTKRDHLHLF PLGYRLWRHT
     KEEAAKGRAS IFDIFRKHHI TGDHGVPLGG IGAGSIGRSY KGEFQQFKLF PKICEEAPIL
     TNQFSVFVSR PGGLKHSTVL CPTKPEVIKD NGGYLCRGEA PNVGIDSWDW NMTGEKSTYH
     ALYPRSWTVY EGEPDPELRI VSRQVSPFIP HNYKETSLPV SVFDFTVTNT GAEQATVTLL
     FTWENSVGGT SGLTGQHLNS TIMAKDGVHA VALHHKTANG HPPVTYAIAA KETEDVRVSS
     CPSFLVSGTS PNKITAGDMW DEIKKNASFD KLTINACSPS KPGTSIGAAI AAKVKVPPGC
     DRTVTFSLSW DCPEARFDEK TYHRRYTRFY GGLGNAAVAM AHDALLKFSE WEAQIEEWQG
     PILADTSLPE WYRTTLFNEL YYFNSGGTIW TDGLPPKQSL DSIGRRKISL SISTIDNPDS
     DQNNIALDIL GRIDAVCSQI HAPLSSNAAL GTTMVQHTEE NIGQFLYLEG IQYLMYNTYD
     VHFYSSFALL MLFPKLELSI QRDFAAAVLM HDSSKKQVMS SGEFVTRKVL GAVPHDIGLN
     DPWFEVNAYN LFNTDRWKDL NSKFVLQVYR DVVATGDIKF AKSVWPSVYT AIAYLDQFDK
     DGDGMIENEG FPDQTYDAWS CTGVSAYCGG LWVAALQAGS ALALLVGDNG AAVYFNAKYE
     KARSVYEKLW NGSYFNYDNS RSGSSSSILA DQLAGQWYAR ACGLKPIAKE EWIKKALETV
     YDFNVMKVRE GTRGAVNGML PDGRVDTSTM VSREVWAGTT YSVAACMIQE GLADKGFRTA
     RGIYEAAWSD RGLGVSFQTP EAWTTNDEYR SLCYMRPLAI WGMQWAHTMP QPNREQEQSL
     RIQEEEETSV LFQQHAGFIK VAHYLKTTKG KDHRSRLQSA YETFLRVVRL
//
DBGET integrated database retrieval system