ID A0A0D3CUW4_BRAOL Unreviewed; 433 AA.
AC A0A0D3CUW4;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=thioglucosidase {ECO:0000256|ARBA:ARBA00012250};
DE EC=3.2.1.147 {ECO:0000256|ARBA:ARBA00012250};
DE AltName: Full=Sinigrinase {ECO:0000256|ARBA:ARBA00032643};
DE AltName: Full=Thioglucosidase {ECO:0000256|ARBA:ARBA00032797};
GN Name=106300052 {ECO:0000313|EnsemblPlants:Bo6g076810.1};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo6g076810.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo6g076810.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo6g076810.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- FUNCTION: Degradation of glucosinolates (glucose residue linked by a
CC thioglucoside bound to an amino acid derivative) to glucose, sulfate
CC and any of the products: thiocyanates, isothiocyanates, nitriles,
CC epithionitriles or oxazolidine-2-thiones.
CC {ECO:0000256|ARBA:ARBA00003014}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a thioglucoside + H2O = a sugar + a thiol.; EC=3.2.1.147;
CC Evidence={ECO:0000256|ARBA:ARBA00034026};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838, ECO:0000256|RuleBase:RU003690}.
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DR RefSeq; XP_013591561.1; XM_013736107.1.
DR AlphaFoldDB; A0A0D3CUW4; -.
DR STRING; 109376.A0A0D3CUW4; -.
DR EnsemblPlants; Bo6g076810.1; Bo6g076810.1; Bo6g076810.
DR GeneID; 106300052; -.
DR Gramene; Bo6g076810.1; Bo6g076810.1; Bo6g076810.
DR KEGG; boe:106300052; -.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_3_0_1; -.
DR OMA; YIDEELY; -.
DR OrthoDB; 3373839at2759; -.
DR Proteomes; UP000032141; Chromosome C6.
DR GO; GO:0102799; F:glucosinolate glucohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0019137; F:thioglucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR10353:SF221; THIOGLUCOSIDASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..433
FT /note="thioglucosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002259036"
SQ SEQUENCE 433 AA; 48218 MW; 4B95CAF9D43CAD2F CRC64;
MRSEGLVFML LITSAYVGVF AKNHSSRPKL RRSDFPQGFI FGCATSAYQC EGAAHEDGRG
PSIWDTFSEK FPEKIMGGSN GSIADDSYNL YKEDVNFLHQ IGFDAYRFSI SWSRILPCGD
LKGGINQAGI DYYNNLINHL LSKGVKPYVT IFHWDLPEAL QHTYGGFLGA EIVNDFRDYA
ELCFQKFGDR VKHWTTLNEP FSVVHNGFTT GQEAPGRCSS FANPNCTGGD GASEPYIVGH
NFLLAHGAAV NIYREKYQAI QKGEIGIALN TVWHYPYSDS YADKLAAARA TAFTFDYFVE
PIVYGKYPTE MVNHVKDGRL PTFTPEESSM LKGSYDFIGI NYYSSSYVKD VPCATENITM
STDACVSIVG ERNGVPLGPT AGSDWLLIYP KGIRDLLLHV KFKFDDPVLY ITENGVDEAS
IGEIFLNDDL RID
//
