ID A0A0D3D4W2_BRAOL Unreviewed; 501 AA.
AC A0A0D3D4W2;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=thioglucosidase {ECO:0000256|ARBA:ARBA00012250};
DE EC=3.2.1.147 {ECO:0000256|ARBA:ARBA00012250};
DE AltName: Full=Sinigrinase {ECO:0000256|ARBA:ARBA00032643};
DE AltName: Full=Thioglucosidase {ECO:0000256|ARBA:ARBA00032797};
GN Name=106301235 {ECO:0000313|EnsemblPlants:Bo7g031500.1};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo7g031500.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo7g031500.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo7g031500.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- FUNCTION: Degradation of glucosinolates (glucose residue linked by a
CC thioglucoside bound to an amino acid derivative) to glucose, sulfate
CC and any of the products: thiocyanates, isothiocyanates, nitriles,
CC epithionitriles or oxazolidine-2-thiones.
CC {ECO:0000256|ARBA:ARBA00003014}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a thioglucoside + H2O = a sugar + a thiol.; EC=3.2.1.147;
CC Evidence={ECO:0000256|ARBA:ARBA00034026};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838, ECO:0000256|RuleBase:RU003690}.
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DR RefSeq; XP_013593042.1; XM_013737588.1.
DR AlphaFoldDB; A0A0D3D4W2; -.
DR STRING; 109376.A0A0D3D4W2; -.
DR EnsemblPlants; Bo7g031500.1; Bo7g031500.1; Bo7g031500.
DR GeneID; 106301235; -.
DR Gramene; Bo7g031500.1; Bo7g031500.1; Bo7g031500.
DR KEGG; boe:106301235; -.
DR eggNOG; KOG0626; Eukaryota.
DR HOGENOM; CLU_001859_1_0_1; -.
DR OMA; NEANACA; -.
DR OrthoDB; 3373839at2759; -.
DR Proteomes; UP000032141; Chromosome C7.
DR GO; GO:0102799; F:glucosinolate glucohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0019137; F:thioglucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR10353:SF254; THIOGLUCOSIDASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..501
FT /note="thioglucosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002259257"
SQ SEQUENCE 501 AA; 56729 MW; 9EAC628F91BD967C CRC64;
MEHVLLLITI LLCLAFSGRC IDAYSRNDFP EGFLFGSAFS AFQWEGAVDE DGRMPSIWDT
YIRSNNAPSG DIACDGYHKY KEDVRLMYDM GLDAFRLSIS WSRLIPGGRG PVNPKGLLFY
KNLIDELTRH GIEPHVTLYH NDLPQALEDE YGGWIDRRII DDFTAFADIC FRELGNKVKF
WSTINEPNMS ALGGYDLGFM PPEHCSAPFG HVNCSRGNSS TEPYIAIHNM LLAHASTARL
YKQKYKGEQN GSVCMTCFSY WVVPFSSSKE DEMATQIAKD FFLGWILHPL VFGDYPDTMK
RLVGKRLPSF SEEETKVVKD SSDFIGVIHY TTMTAAHVST FQQGDFSADM NALVSPFGNS
TLVKYDVLPW GLEGVLAYIK ENYGNPPVYI LENGQSTNHY SSLDDVERVE YLQAYIGAVL
DSVRNGSKTR GYFQWSFMDL YEFLDTNYTY GLYYVNFSHP ERKRSPKTSA FWYSAFLKGM
IVSSQDMKNL SVSSSTGFSS Q
//
