ID A0A0D3D8H5_BRAOL Unreviewed; 1198 AA.
AC A0A0D3D8H5;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo7g064920.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo7g064920.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo7g064920.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641}.
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DR AlphaFoldDB; A0A0D3D8H5; -.
DR STRING; 109376.A0A0D3D8H5; -.
DR EnsemblPlants; Bo7g064920.1; Bo7g064920.1; Bo7g064920.
DR Gramene; Bo7g064920.1; Bo7g064920.1; Bo7g064920.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_007823_0_0_1; -.
DR OMA; NSMMNMY; -.
DR Proteomes; UP000032141; Chromosome C7.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 5.
DR InterPro; IPR046848; E_motif.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR InterPro; IPR002885; Pentatricopeptide_rpt.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR NCBIfam; TIGR00756; PPR; 5.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR PANTHER; PTHR31451:SF60; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR Pfam; PF20431; E_motif; 1.
DR Pfam; PF01535; PPR; 5.
DR Pfam; PF12854; PPR_1; 1.
DR Pfam; PF13041; PPR_2; 2.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51375; PPR; 4.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00023295};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..1198
FT /note="mannan endo-1,4-beta-mannosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002260085"
FT DOMAIN 71..336
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
FT REPEAT 464..498
FT /note="PPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00708"
FT REPEAT 765..799
FT /note="PPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00708"
FT REPEAT 866..900
FT /note="PPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00708"
FT REPEAT 968..1002
FT /note="PPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00708"
SQ SEQUENCE 1198 AA; 132800 MW; A93B8ABF4CB5C69D CRC64;
MKPLCLVTIL SILIQQSYLK LGADAFSRDG FVRTKGVQFS LNGYPYYANG FNAYWLMYVA
SDPSQRPKIS AAFQEASRHG LTVARTWAFS DGGYRPLQYS PGSYNEDMFQ GLDFAIAEAR
RHGIKIILSF ANNYVSFGGK KQYVDWARSR GRPVSSEDDF FTDFLVKDFY KNHIKAVLNR
FNTLTKVHYR DDPTIMAWEL MNEPRCPSDP TGRTIQAWIT EMAAHVKSLD RNHLLEAGLE
GFYGQSSPQS KTLNPPGQFG TDFIANNRIP GIDFVTVHSY PDEWFVDSSE QSQMEFLNKW
LDAHIQDAQN VLHKPIILAE FGKSTKKAGS AQRDAVFNTV YGKIYESAKR GGAAAGGLFW
QLLGNGMDNF QDGYGIILSQ SSSTVNVIAQ QSRKLTLIRR IFARMINVEK WKRARGYGPV
RKGASVEKPA YLVSNQLNDQ LKTLVGSGKL RDARQVFDKM PHRDVYSWTT IIQGYIAATN
YDEALSLFSA MHVDDPRVSA DSHVLSVALK ACGQTSNISF GESLHAFAHK THLLSDVYVA
SSLLNLYKGN GMIDESCRLF SELPYRNTVT WTTIITGLVH AGRHKEGLRA PLSQSPKHLS
HSSSSLPLSR GYLCNNNKAE SSSSTILLLF DQSRFCSNPP TASSQSGTGF ITVALDVEIV
PPENSVLHTA AERTPNMGLC NNHALMYFSE MSRFEGLPDT FTFAIALKAC AGLRQVQYGR
GIHTHVISKG FGAVLDVANS LFTMYTECGE MEDGLRLFES MRERDVVSWT SLITAYSRIG
QEENAVKTFL EMRNSDVPPN EQTFASAFAA CASLSRLVWG EQLHGNVISL GLGDYLSVSN
SMMNMYSKCG ELDSASALFR GMRCRDIISW STIIGGYSQG GLPEEGFECF SWMRRSGTKP
TDFALASLLS VSGNMAVLEQ GRQVHALALY LGLEQSPTVR SALINMYSKC GSIVEASKVF
KETERGADIV ALTAMINGYA EHGKSKEAID LFEKSLKVGF RPDAVSFISV LTACSHSGQL
GLGFHYFNLM QDKYNMSPAK EHYGCMVDLL CRAGRLSEAE KMIDEMPFEK DDVVWTTLLR
ACTAKRDVER GRRAAERVLE VEPTSSTALV TLANIYSSTE KWKEAAIVRK SMKSKGVVKE
PGWSSILIKD RVSAFASGGR SHPQSEDIYS VLESLVSGAE PLRYGCEMKR DSGSIQIL
//
