ID A0A0D3DC84_BRAOL Unreviewed; 1124 AA.
AC A0A0D3DC84;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 08-NOV-2023, entry version 43.
DE RecName: Full=Cytosine-specific methyltransferase {ECO:0000256|RuleBase:RU000417};
DE EC=2.1.1.37 {ECO:0000256|RuleBase:RU000417};
GN Name=106303172 {ECO:0000313|EnsemblPlants:Bo7g092860.1};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo7g092860.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo7g092860.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo7g092860.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37;
CC Evidence={ECO:0000256|RuleBase:RU000417};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. C5-methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01016, ECO:0000256|RuleBase:RU000416}.
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DR RefSeq; XP_013594964.1; XM_013739510.1.
DR AlphaFoldDB; A0A0D3DC84; -.
DR STRING; 109376.A0A0D3DC84; -.
DR EnsemblPlants; Bo7g092860.1; Bo7g092860.1; Bo7g092860.
DR GeneID; 106303172; -.
DR Gramene; Bo7g092860.1; Bo7g092860.1; Bo7g092860.
DR KEGG; boe:106303172; -.
DR eggNOG; ENOG502QPKK; Eukaryota.
DR HOGENOM; CLU_002247_0_0_1; -.
DR OMA; ISIRTNV; -.
DR OrthoDB; 317994at2759; -.
DR Proteomes; UP000032141; Chromosome C7.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IEA:InterPro.
DR GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd04708; BAH_plantDCM_II; 1.
DR Gene3D; 2.30.30.490; -; 2.
DR Gene3D; 3.90.120.10; DNA Methylase, subunit A, domain 2; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR001025; BAH_dom.
DR InterPro; IPR043151; BAH_sf.
DR InterPro; IPR018117; C5_DNA_meth_AS.
DR InterPro; IPR001525; C5_MeTfrase.
DR InterPro; IPR031303; C5_meth_CS.
DR InterPro; IPR022702; Cytosine_MeTrfase1_RFD.
DR InterPro; IPR017198; DNMT1-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00675; dcm; 1.
DR PANTHER; PTHR10629; CYTOSINE-SPECIFIC METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10629:SF52; DNA (CYTOSINE-5)-METHYLTRANSFERASE 1; 1.
DR Pfam; PF01426; BAH; 2.
DR Pfam; PF00145; DNA_methylase; 2.
DR Pfam; PF12047; DNMT1-RFD; 1.
DR PIRSF; PIRSF037404; DNMT1; 4.
DR PRINTS; PR00105; C5METTRFRASE.
DR SMART; SM00439; BAH; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51038; BAH; 2.
DR PROSITE; PS00094; C5_MTASE_1; 1.
DR PROSITE; PS00095; C5_MTASE_2; 1.
DR PROSITE; PS51679; SAM_MT_C5; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01016}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01016};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01016}.
FT DOMAIN 329..466
FT /note="BAH"
FT /evidence="ECO:0000259|PROSITE:PS51038"
FT DOMAIN 514..642
FT /note="BAH"
FT /evidence="ECO:0000259|PROSITE:PS51038"
FT REGION 286..307
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 786
FT /evidence="ECO:0000256|PIRSR:PIRSR037404-1,
FT ECO:0000256|PROSITE-ProRule:PRU01016"
SQ SEQUENCE 1124 AA; 128026 MW; 3EB82683CF637649 CRC64;
MEEESSNGHV VNQPRRIVHN WALYNSETRL ISLELLPMMR PTDETIFGAG LLAEDDGSSW
FCLDGSDIST QSHDCKGIPI YLNQIKEWMI EYDSSMVSIS IRTNVAWYRL GKPSTQYAPW
HEPILKTARV AFSIISLLVK QARMARLSFR DVIKRVSQLQ KNDNKAYISS DPSAVERYLV
LHGKVILQIF AKHPKKELKK CAFITGLASK MEERHHTKWA IKKRRKILPK KPKNKILPKK
PKRKLRIGKG YVASKRSAMQ ATKTHLVDRI WGQYYSEFEA IGAENGKEKV QEERQNEEEN
DVEEPSVMET RWEGDILGRT SAGEPLYQQA LVGGEVVAVG GAVLLEVNET SVIYFVEYMF
ESSDHCKMLH GRHLQRGSET VLGNAANERE LFLTNACMTV QLKDIKGTVS FEIRSKPWGH
QYRKENNAAD ELDRARAEER KAQHLPAEYF CKCLYSPVRG GFFSLQPNDD ISSGSGSCTS
CKMREEKNER SKPKLNAKKT RFVFNGVDYC MEDYAYVNHK FFYKPEEEKK FETNVDSKSF
VVCQLLKVVV SKVSSLNDLF EVIVRRFYRP EDMSPDKAFT SDIQEVYYSE DVYNLPPGAF
EGKCEVRKKH DMPLSNDYLT LDNIFFCELF YDSSRDSLYQ MPVHIKPKFS SIKDDTLLRK
LKGEGIEGET DYDELPKEMR LATLDIFAGC GGLSKGLEQS GVSETKWAIE YEEPAGQAFK
QNHPESTVFV NNCNVILRAI MEKCGDKDEC LSTTEANELA AKLDEEQKLA LPLPGQVDFI
NGGPPCQGFS GMNRFQEGSW SKVQREMILA FLSFAEYFRP RYFLLENVRN FVSFNGGQTF
KLTLASLLEM GYQVRFGVLE AGAYGVSQSR KRAFIWAAAP GEVLPEWPEP MHVFGTPELK
IKLSKRSHYA AVRSTQYGAP FRSITVKDTI NDLPPIENGE SETNREYGAD PVSWFQKKIR
GNMFVLTDHI CKEMDETNLI RCKNIPKTPG ADWRDLQNLK KKKIKLSSGK MVDMIPAWLR
NKAKTHNGWK GIFGRLDMEG SFATSITDPR PMGMVGMCFH PEQDRIISVR ECARSQGFPD
SYEFAGKILD KHRQIGNAVP PPLAFALGRK LKEAVLLKKL PLQQ
//
