ID A0A0D3DYY2_BRAOL Unreviewed; 560 AA.
AC A0A0D3DYY2;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=NEDD8-activating enzyme E1 regulatory subunit {ECO:0000256|PIRNR:PIRNR039099};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo8g115290.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo8g115290.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo8g115290.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- FUNCTION: Regulatory subunit of the dimeric E1 enzyme. E1 activates
CC RUB1/NEDD8 by first adenylating its C-terminal glycine residue with
CC ATP, thereafter linking this residue to the side chain of the catalytic
CC cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally
CC transfers RUB1 to the catalytic cysteine of RCE1.
CC {ECO:0000256|PIRNR:PIRNR039099}.
CC -!- PATHWAY: Protein modification; protein neddylation.
CC {ECO:0000256|ARBA:ARBA00005032, ECO:0000256|PIRNR:PIRNR039099}.
CC -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family. ULA1
CC subfamily. {ECO:0000256|ARBA:ARBA00006868,
CC ECO:0000256|PIRNR:PIRNR039099}.
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DR AlphaFoldDB; A0A0D3DYY2; -.
DR STRING; 109376.A0A0D3DYY2; -.
DR EnsemblPlants; Bo8g115290.1; Bo8g115290.1; Bo8g115290.
DR Gramene; Bo8g115290.1; Bo8g115290.1; Bo8g115290.
DR eggNOG; KOG2016; Eukaryota.
DR HOGENOM; CLU_019618_2_1_1; -.
DR OMA; TMEHSHI; -.
DR UniPathway; UPA00885; -.
DR Proteomes; UP000032141; Chromosome C8.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019781; F:NEDD8 activating enzyme activity; IEA:UniProtKB-UniRule.
DR GO; GO:0045116; P:protein neddylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.40.50.12550; Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2; 1.
DR InterPro; IPR030667; APP-BP1.
DR InterPro; IPR045886; ThiF/MoeB/HesA.
DR InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR InterPro; IPR035985; Ubiquitin-activating_enz.
DR PANTHER; PTHR10953:SF244; NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT AXR1; 1.
DR PANTHER; PTHR10953; UBIQUITIN-ACTIVATING ENZYME E1; 1.
DR Pfam; PF00899; ThiF; 1.
DR PIRSF; PIRSF039099; APP-BP1; 2.
DR SUPFAM; SSF69572; Activating enzymes of the ubiquitin-like proteins; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|PIRNR:PIRNR039099}.
FT TRANSMEM 430..454
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 17..538
FT /note="THIF-type NAD/FAD binding fold"
FT /evidence="ECO:0000259|Pfam:PF00899"
SQ SEQUENCE 560 AA; 62555 MW; A24833F665C6381A CRC64;
MKRKKEEATM SEPKTKYDRQ LRIWGELGQA ALEEASICLL NCGPTGSEAL KNLVLGGVGS
FTVVDGSKVE LGDLGNNFMV DDERSVGQSK AKSVCAFLQE LNDSVKATFI EENPDTLIST
NPFFFSQFTL VIATQLVEDS MVKLDRICRE ANVMLVFARS YGLSGFVRVS VKEHTVIDSK
PDHFLDDLRL NNPWPELKSF VETIDLNVSD PVAHKHIPYV VILVKVADEW TRSHSVQDDI
YGLWMRITTK KLSKQLSKFL LRVESALRFN NDTCAEPNSN SSDFWVMVAA LKEFVSNEGD
GEAPLEGSIP DMTSSTELSA TASLHCCFST DTIYQSKAEA DFLVMEKRVK NILKKIGRDL
SSISKPTIKS FCKNARKLKV CRYRMVEDEF SNPCVTEIQK CLADEDYRWQ CLHGVCPFHM
NTKASNFERL LFYGSSYAYI VYCGSGAMGF YVLLRAVDRF TANYNKFPGQ FDGGMDEDIS
RLKTTALSLL ADLGCNGSVL PDDLINEMCR FGASELHVVA AFIGGIASQE VIKLVTKQFV
PMLGTYIFNG IDHKSQLLTL
//
