ID A0A0D3EDL6_BRAOL Unreviewed; 726 AA.
AC A0A0D3EDL6;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo9g150240.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo9g150240.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo9g150240.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
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DR AlphaFoldDB; A0A0D3EDL6; -.
DR STRING; 109376.A0A0D3EDL6; -.
DR EnsemblPlants; Bo9g150240.1; Bo9g150240.1; Bo9g150240.
DR Gramene; Bo9g150240.1; Bo9g150240.1; Bo9g150240.
DR eggNOG; KOG0987; Eukaryota.
DR HOGENOM; CLU_001324_0_1_1; -.
DR OMA; MTYLVEY; -.
DR Proteomes; UP000032141; Chromosome C9.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR025476; Helitron_helicase-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR Pfam; PF14214; Helitron_like_N; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT DOMAIN 1..89
FT /note="Helitron helicase-like"
FT /evidence="ECO:0000259|Pfam:PF14214"
FT DOMAIN 548..594
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 726 AA; 82241 MW; 7B6F7694711D6AAE CRC64;
MAICRWYGNP DLFITITTNP KWDETSDHLK MYGSDDPNDR PDLEARVFKM KLDELISDFN
KGIFFPRPKA VVYTIEFQKR GLPHAHILLW LDGDFRNPTA SDIDKIISAE LPDKEKDPEA
YGLVEQHMMH GPCGEDRKAS PCMEDDPEKM QQRRLLRFPD LQLSPNELQQ YTLIEIECLL
QNFEKSLTEF TVMPLPNKAV MDQIKHKAMA RHDQFDIAEE AIIHEKLFDK LNHQQRAVYD
AVIKSVFQKE GRLFFLYGAG GTGKTFLYRA IIAALRSSSK KVIPVASSAI AALLLPGGRT
AHSRFNIPLK LFEDTYCEVK AGTILANFLN ESDLIIWDEA PMAHRHTFEA VDRTLRDILA
VTDKTTLTEP FGGKTVLLGG DFRQILPVIP QGTRQDTVNA ALNRSHLWNN CEIFLLTQNM
RVKPEEKAFA DWILEVGDGR AAKEPQILDD CAQPEDQILI DKTILLPITS TPLETLCSSA
FPDFANDHKD LYKIRETAIL TPRNVTVDEI NTYLVSKVPG EEKEYLSADS FAEEEQHSGD
LDMTYLVEYL NSLEFPGLPA HKLRLKVGVP VMLLHNLNQK EGLCNGTRLI VTHLGEKVIK
TEMLTSTTKR DPILLPRIIL SPPESNHPFT LKRRQFPIRV CYAMTINKSQ GQTLSHVSLY
LPKPVFSHGQ LYVALSRVTT PKGLKILDLS RDGDESRTIS NIVYREAYNG LPQSTGSDDD
ETSKDQ
//
