ID   A0A0D3GMM1_9ORYZ        Unreviewed;       949 AA.
AC   A0A0D3GMM1;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   RecName: Full=Small RNA 2'-O-methyltransferase {ECO:0000256|ARBA:ARBA00021330};
DE            EC=2.1.1.386 {ECO:0000256|ARBA:ARBA00035025};
OS   Oryza barthii.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=65489 {ECO:0000313|EnsemblPlants:OBART07G04060.2};
RN   [1] {ECO:0000313|EnsemblPlants:OBART07G04060.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IRGC 105608 {ECO:0000313|EnsemblPlants:OBART07G04060.2};
RX   DOI=10.1007/s12284-009-9025-z;
RA   Rounsley S., Marri P.R., Yu Y., He R., Sisneros N., Goicoechea J.L.,
RA   Lee S.J., Angelova A., Kudrna D., Luo M., Affourtit J., Desany B.,
RA   Knight J., Niazi F., Egholm M., Wing R.A.;
RT   "De novo next generation sequencing of plant genomes.";
RL   Rice 2:35-43(2009).
RN   [2] {ECO:0000313|EnsemblPlants:OBART07G04060.2}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (MAR-2015) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H(+) +
CC         S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide;
CC         Xref=Rhea:RHEA:37887, Rhea:RHEA-COMP:10415, Rhea:RHEA-COMP:10416,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74896, ChEBI:CHEBI:74898; EC=2.1.1.386;
CC         Evidence={ECO:0000256|ARBA:ARBA00034992};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family.
CC       {ECO:0000256|ARBA:ARBA00009026}.
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DR   AlphaFoldDB; A0A0D3GMM1; -.
DR   STRING; 65489.A0A0D3GMM1; -.
DR   PaxDb; 65489-OBART07G04060-2; -.
DR   EnsemblPlants; OBART07G04060.2; OBART07G04060.2; OBART07G04060.
DR   Gramene; OBART07G04060.2; OBART07G04060.2; OBART07G04060.
DR   eggNOG; KOG1045; Eukaryota.
DR   Proteomes; UP000026960; Chromosome 7.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProtKB-KW.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 3.30.160.20; -; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR026610; Hen1.
DR   InterPro; IPR040870; HEN1_dsRBD2.
DR   InterPro; IPR040813; Hen1_Lam_C.
DR   InterPro; IPR013217; Methyltransf_12.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR21404; HEN1; 1.
DR   PANTHER; PTHR21404:SF3; SMALL RNA 2'-O-METHYLTRANSFERASE; 1.
DR   Pfam; PF17842; dsRBD2; 1.
DR   Pfam; PF18441; Hen1_Lam_C; 1.
DR   Pfam; PF21224; Hen1_LCD; 1.
DR   Pfam; PF08242; Methyltransf_12; 1.
DR   SUPFAM; SSF54768; dsRNA-binding domain-like; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW   Reference proteome {ECO:0000313|Proteomes:UP000026960};
KW   RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          233..369
FT                   /note="Small RNA 2'-O-methyltransferase Hen1 La-motif C-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF18441"
FT   DOMAIN          371..509
FT                   /note="HEN1 double-stranded RNA binding"
FT                   /evidence="ECO:0000259|Pfam:PF17842"
FT   DOMAIN          724..820
FT                   /note="Methyltransferase type 12"
FT                   /evidence="ECO:0000259|Pfam:PF08242"
SQ   SEQUENCE   949 AA;  104638 MW;  0F905E3DCCE468D6 CRC64;
     MPLRLRGGGG SKRGMPAAPT VTPKAVIHQK YGAKACYSVE EVREAVDGGC PGLALPQQTR
     SVYRCSLDLP GLTVVTPGTF VRKKDAEQAA AQIALDKLGI QPTANAPSTP EEAWDELIAR
     ISGFFADENF PSSSHPLIGH MCVTFRRTGD RFGMIPMSAI AACDVKVIGL CKLIDPKAEF
     DPLLVLSLIY NAAKKSPGVS VSDSNFWIRS QKPYSPEAVD LALQHWSGIT NPIEVDGIFV
     PCVMEDEPKT IRLTLSHNEH YMGDIVSKLS ASDSSHAVVS RTVGKASSEI RLYFSAPNVQ
     FVSEISNNVV SSLGDGYMES LINKRASFIS GQTIYGDAIL ANVGYTRRDS ELHTEDVTLS
     NYYRILLGKS PDGNYKISRD SILVAELPSV YSRSSWKGLS PRDLLCSFCR LHRLAEPYFV
     VNRVSASCKV LGSHVSSEEM DVLKNTENQC ASGGKNDKEN PDMFKCDVKI YSKKQELLLE
     YSTADTWSKE SDAIHNSSLK VLIWFCSYFK QPNKHVLKLS HSKSTDGFTI CPDNFLHEFA
     MFLSIYGNTG GDDSSTCSTV GSLSMDTSKQ KLENNAVLAH VDGPDSGVFP SHGSLTCISY
     TASLVVKDKT NRYMLESNNE FEFEIGTGAV KNQIESCVSQ LSVNQSACFI AELPPRDLIL
     AAANEFSHDL SKISRDNCFL EFSVKVTEPL EDRMEKALFN PPLSKQRVEF AVRYINELHA
     TTLVDFGCGS GSLLDSLLEH PTTLEEVVGV DISRKGLTRA AKSLHQKLSK KSLMQTSVPT
     AVLYDGSITD FDSRLYRFDI GTCLEVIEHV EEDQASLFGN VVLSSFCPTV LIVSTPNYEY
     NPILQRSAMP NKEEEPEENA GPCKFRNHDH KFEWTRSQFQ HWATGLAEKH NYSVEFSGVG
     GSGDEPGFAS QIAVFRRMAS GQDEVCQEGE LHQPYELLWE WPNASLPSH
//