ID A0A0D3GQN3_9ORYZ Unreviewed; 438 AA.
AC A0A0D3GQN3;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=Glutamate--cysteine ligase {ECO:0000256|PIRNR:PIRNR017901};
DE EC=6.3.2.2 {ECO:0000256|PIRNR:PIRNR017901};
OS Oryza barthii.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=65489 {ECO:0000313|EnsemblPlants:OBART07G13360.1};
RN [1] {ECO:0000313|EnsemblPlants:OBART07G13360.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IRGC 105608 {ECO:0000313|EnsemblPlants:OBART07G13360.1};
RX DOI=10.1007/s12284-009-9025-z;
RA Rounsley S., Marri P.R., Yu Y., He R., Sisneros N., Goicoechea J.L.,
RA Lee S.J., Angelova A., Kudrna D., Luo M., Affourtit J., Desany B.,
RA Knight J., Niazi F., Egholm M., Wing R.A.;
RT "De novo next generation sequencing of plant genomes.";
RL Rice 2:35-43(2009).
RN [2] {ECO:0000313|EnsemblPlants:OBART07G13360.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC Evidence={ECO:0000256|PIRNR:PIRNR017901};
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2. {ECO:0000256|ARBA:ARBA00005006}.
CC -!- SUBUNIT: Homodimer or monomer when oxidized or reduced, respectively.
CC {ECO:0000256|ARBA:ARBA00011153}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229, ECO:0000256|PIRNR:PIRNR017901}.
CC -!- SIMILARITY: Belongs to the carboxylate-amine ligase family.
CC Glutamate--cysteine ligase type 2 subfamily.
CC {ECO:0000256|ARBA:ARBA00010253, ECO:0000256|PIRNR:PIRNR017901}.
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DR STRING; 65489.A0A0D3GQN3; -.
DR PaxDb; 65489-OBART07G13360-1; -.
DR EnsemblPlants; OBART07G13360.1; OBART07G13360.1; OBART07G13360.
DR Gramene; OBART07G13360.1; OBART07G13360.1; OBART07G13360.
DR eggNOG; ENOG502QVEN; Eukaryota.
DR HOGENOM; CLU_026610_1_0_1; -.
DR UniPathway; UPA00142; UER00209.
DR Proteomes; UP000026960; Chromosome 7.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR GO; GO:0052544; P:defense response by callose deposition in cell wall; IEA:EnsemblPlants.
DR GO; GO:0042742; P:defense response to bacterium; IEA:EnsemblPlants.
DR GO; GO:0050832; P:defense response to fungus; IEA:EnsemblPlants.
DR GO; GO:0002213; P:defense response to insect; IEA:EnsemblPlants.
DR GO; GO:0009908; P:flower development; IEA:EnsemblPlants.
DR GO; GO:0019761; P:glucosinolate biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009700; P:indole phytoalexin biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0046686; P:response to cadmium ion; IEA:EnsemblPlants.
DR GO; GO:0009408; P:response to heat; IEA:EnsemblPlants.
DR GO; GO:0009753; P:response to jasmonic acid; IEA:EnsemblPlants.
DR GO; GO:0010193; P:response to ozone; IEA:EnsemblPlants.
DR Gene3D; 3.30.590.20; -; 1.
DR InterPro; IPR035434; GCL_bact_plant.
DR InterPro; IPR006336; GCS2.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR011556; Glut_cys_lig_pln_type.
DR NCBIfam; TIGR01436; glu_cys_lig_pln; 1.
DR PANTHER; PTHR34378; GLUTAMATE--CYSTEINE LIGASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR34378:SF1; GLUTAMATE--CYSTEINE LIGASE, CHLOROPLASTIC; 1.
DR Pfam; PF04107; GCS2; 1.
DR PIRSF; PIRSF017901; GCL; 1.
DR SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|PIRNR:PIRNR017901};
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528, ECO:0000256|PIRNR:PIRNR017901};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157,
KW ECO:0000256|PIRSR:PIRSR017901-50};
KW Glutathione biosynthesis {ECO:0000256|ARBA:ARBA00022684};
KW Ligase {ECO:0000256|PIRNR:PIRNR017901};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR017901};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000026960};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DISULFID 102..322
FT /evidence="ECO:0000256|PIRSR:PIRSR017901-50"
SQ SEQUENCE 438 AA; 50277 MW; F05409CE14BD24E4 CRC64;
MTEPLTKEDL MAYLVSGCKP KENWRIGTEH EKFGFEVDTL XPIKYDQIRD ILNGLAERFD
WDKIVEENNV IGLKQGKQSI SLEPGGQFEL SGAPLETLHQ TCAEVNSHLY QVKAVGEEMG
IGFLGIGFQP KWALSDIPIM PKGRYEIMRN YMPKVGSLGL DMMFRTCTVQ VNLDFSSEQD
MIRKFRAGLA LQPIATAIFA NSPFKEGKPN GYLSLRSHIW TDTDNNRSGM LPFVFDDSFG
FERYVDYALD VPMYFVYRNK KYIDCTGMSF RDFMVGKLPQ APGDLPTLND WENHLTTIFP
EVRLKRYLEM RGADGGPWRR LCALPAFWVG LLYDEESLQS ISDMTSDWTN EEREMLRRKV
PVTGLKTPFR DGYVRDLAEE ILQLSKNGLE RRGYKEVGFL REVDAVISSG VTPAERLLNL
YETKWQRSVX PVFQELLY
//
