ID   A0A0D3HEN0_9ORYZ        Unreviewed;       951 AA.
AC   A0A0D3HEN0;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   22-FEB-2023, entry version 25.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS   Oryza barthii.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=65489 {ECO:0000313|EnsemblPlants:OBART10G13010.2};
RN   [1] {ECO:0000313|EnsemblPlants:OBART10G13010.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IRGC 105608 {ECO:0000313|EnsemblPlants:OBART10G13010.2};
RX   DOI=10.1007/s12284-009-9025-z;
RA   Rounsley S., Marri P.R., Yu Y., He R., Sisneros N., Goicoechea J.L.,
RA   Lee S.J., Angelova A., Kudrna D., Luo M., Affourtit J., Desany B.,
RA   Knight J., Niazi F., Egholm M., Wing R.A.;
RT   "De novo next generation sequencing of plant genomes.";
RL   Rice 2:35-43(2009).
RN   [2] {ECO:0000313|EnsemblPlants:OBART10G13010.2}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (MAR-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
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DR   AlphaFoldDB; A0A0D3HEN0; -.
DR   EnsemblPlants; OBART10G13010.2; OBART10G13010.2; OBART10G13010.
DR   Gramene; OBART10G13010.2; OBART10G13010.2; OBART10G13010.
DR   Proteomes; UP000026960; Chromosome 10.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 2.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000026960}.
FT   DOMAIN          102..401
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          537..895
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
FT   REGION          85..105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          444..477
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        463..477
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   951 AA;  106648 MW;  AF9198E5CBE10B66 CRC64;
     MVSGNLFHCR RNSWPAEEYV GRTALQLLDF DGGSPPEQAW RRRLNSHANL LKEFSVTFME
     AMRMMSLGLR LWSYVREEAS HGRKAPIDPF TKEKCKPSAS QGVPLGGMGS GSISRGFRGE
     FKNWHIIPGL CETSPIFVSR DGGNKKYSSV LSPGHHEGLK KCNDSGISSW DWNLSGQHST
     YHALFPRAWT VYDGEPDPDL KISCRQISPF IPHDYKDSSL PTSVFVYTLV NTGKDRAKVS
     LLMTWANSIG GFSHHSGGHF NEPFITAKDN PPVTFAIAAC ETQNVNVTVL PVFGLSGEGH
     DSAKQMWDRM KQNGHFDREN FEAGTSMPSS SGETLCAAVS ASTWVEPHGR CTVVFGLAWS
     SPKIKFQKGC TYNRRYTEFY GTSERSAVNL VHDALTKYRI WEEEIEKWQN PILKNEKLPE
     WYKFTLFNEL YFLVAGGTVW TDGQPPVIDG KPSPGSNQQK SSKRGTRDTK QESVKDNHVK
     LTAEQVTNGG DLANGEEQSV SKYAAVHGPQ MAKATNGLGS QEPIPYLLSK NGPENVGKFL
     YLEGVEYIMW CTYDVHFYAS FALLDLFPKI ELSIQRDFAN AVLYEDRRRM KFLADGTSGI
     RKVKGAVPHD LGTHDPWHEM NAYNIHDTSK WKDLNPKFVY RDFAATGDMS FGRDVWPAVC
     AAMDYMNQFD RDGDGLIEND GFPDQTYDAW TVHGISAYCG GLWLAALQAA ATMAHRLGDR
     PFAEKYKLKF IQAKAVYEAK LWNGSYFNYD SGTSSNSRSI QADQLAGQWY AASSGLPPLF
     DENKIRSALQ KIFEFNVMKV KGGRLGAVNG MTPNGKVDET CMQSREIWTG VTYGVAANML
     LHGMEHQGFT TAEGIFIAGW SEEGYGYWFQ TPEGWTIDGH YRSLIYMRPL AIWAMQWARS
     PPKAILDAPK VNLMDRIHLS PQMIRAMNEI NVRKIAPDNR CFPSSAFRCE C
//