ID A0A0D5ACE1_9NOCA Unreviewed; 825 AA.
AC A0A0D5ACE1;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=NY08_2427 {ECO:0000313|EMBL:AJW40453.1};
OS Rhodococcus sp. B7740.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Rhodococcus.
OX NCBI_TaxID=1564114 {ECO:0000313|EMBL:AJW40453.1, ECO:0000313|Proteomes:UP000032410};
RN [1] {ECO:0000313|EMBL:AJW40453.1, ECO:0000313|Proteomes:UP000032410}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B7740 {ECO:0000313|EMBL:AJW40453.1};
RX PubMed=25931596;
RA Zhang D., Li L., Zhu S., Zhang N., Yang J., Ma X., Chen J.;
RT "Complete Genome Sequence of Rhodococcus sp. B7740, a Carotenoid-Producing
RT Bacterium Isolated from the Arctic Sea.";
RL Genome Announc. 3:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP010797; AJW40453.1; -; Genomic_DNA.
DR RefSeq; WP_032395773.1; NZ_CP010797.1.
DR AlphaFoldDB; A0A0D5ACE1; -.
DR STRING; 1564114.NY08_2427; -.
DR KEGG; rhb:NY08_2427; -.
DR PATRIC; fig|1564114.4.peg.2388; -.
DR HOGENOM; CLU_004585_5_2_11; -.
DR Proteomes; UP000032410; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 2.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 21..316
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 317..632
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 42..49
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 825 AA; 89431 MW; 28EEB322035646DF CRC64;
MNTFLQGLQS RQNTSSDTLL EGLNPQQREA VVHTGSPLLI VAGAGSGKTA VLTRRIAYLL
AERGVSPGQV LAITFTNKAA AEMRERVAGL VGPRANAMWV STFHSSCVRI LRAQAALLPG
LNSNFSIYDA DDSRRLLTMI SKDLQIDTKK FSARLLSTAI SNLKNELIGP DKAAEDADGD
PAELPRLVAK VYGIYQQRLR AANAMDFDDL IGETVGILQA FPQVAQYYRR RFRHVLVDEY
QDTNHAQYML VRELVGTPDA DGALEGTVAP GELCVVGDAD QSIYAFRGAT IRNIEEFERD
YPDARTILLE QNYRSTQNIL SAANAVISRN IGRREKKLWT DTGEGELIVG YVADNEHDEA
SFVASEIDRL VDGGDVRFDE VAVFYRTNNS SRALEEIFIR LGLPYKVVGG VRFYERKEVR
DLVAYLRVLS NEDDTVSMRR ILNTPRRGIG DRAEACVAVH AEQRDISFSR ALHDAAAGKV
ALLNSRQQKL IAQFMELLDE LRAVLTATDG DGNDIADIGD VVEAVLDRTG YRAELEASSD
PQDGARLDNL NELVSVAREF TSEARNQAAV AAEALETGDD ESGDEDGGVD GLAEPGSLAA
FLERVSLVAD TDQIPDSGTG VVTLMTLHTA KGLEFPVVFV TGWEDGQFPH MRALGDPNEL
SEERRLAYVG ITRARQRLYL TRAIMRSAWG QPIANPESRF LQEVPQHLID WRREDPGTGG
GRAIGGGRSG GYGSGGGYGS NSYGSGGYGG SSNRGQSSTP SFGAAKGRNS NLVLAVGDRV
SHDKYGLGTV LESTGAGVKA MVLIDFGSAG RVKMMLIGGV PMTKL
//
