ID A0A0D5M4L7_9GAMM Unreviewed; 737 AA.
AC A0A0D5M4L7;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=KO116_02616 {ECO:0000313|EMBL:AJY51091.1};
OS Halomonas sp. KO116.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=1504981 {ECO:0000313|EMBL:AJY51091.1, ECO:0000313|Proteomes:UP000028645};
RN [1] {ECO:0000313|EMBL:AJY51091.1, ECO:0000313|Proteomes:UP000028645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KO116 {ECO:0000313|EMBL:AJY51091.1,
RC ECO:0000313|Proteomes:UP000028645};
RX PubMed=25953187;
RA O'Dell K.B., Woo H.L., Utturkar S., Klingeman D., Brown S.D., Hazen T.C.;
RT "Genome Sequence of Halomonas sp. Strain KO116, an Ionic Liquid-Tolerant
RT Marine Bacterium Isolated from a Lignin-Enriched Seawater Microcosm.";
RL Genome Announc. 3:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP011052; AJY51091.1; -; Genomic_DNA.
DR RefSeq; WP_035566174.1; NZ_CP011052.1.
DR AlphaFoldDB; A0A0D5M4L7; -.
DR STRING; 1504981.KO116_02616; -.
DR KEGG; hak:KO116_02616; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_6_2_6; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000028645; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 1..289
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 290..550
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 737 AA; 82422 MW; 12D8274D10D458D8 CRC64;
MKLTAEQQAV VNHTAGHARV AAVAGAGKTT TMAARVLHLL ARGVPQKRIL VLMFNRSAKD
DFQRRLASMA PAGQALPDVR TFHSLGHRLT QSLCRWGALA PRQLLSADWQ LERLLRQASL
NVLRDAVERR DAALEGDRLE TLAHFCGLVK AEMITPESLY ERLNVDPDTD YFPAAFAEAE
RLLESEGVMT YADLLYRPLQ ALEEDSALRG RVEGFLDHVI IDEYQDINAA QQRLLSVLVG
SRAEVMAVGD ANQCIYEWRG AKPDTMLENF TATFGEATDY PLSTTFRHGH ALALTANHAI
AANQRRPNQL CLAAPNNPET RVSVGQGSRL LLDALMDWKA QGRAFNEASL LVRSWALSVP
FQLALLQAGI PFRLQREDRF VFRLPLVQAL AGYLKLSRRP DLLRDPQQLL LLLSQPTPFV
ARERLQRLAH QLATTQQWPE RHDPMLTALK PIQRRTLKKR WLLLCELPQL SAWPPAKLLR
HVVETIEAEK TLKRAAARRD KGEEDVRLLD VLIEQAQSVQ DPDAFIELLE RPVENQAGGV
LISTVHGAKG LEWPLVAVVG VNEEDFPHYS RDNPLSDERL EEERRLFYVA ITRAQEQLLV
LHDGGVHRPS RFIAESAWQD SMRVASCLAN PCAPEASLQV TSVGLVERYL TRLGRDDIVL
AAAHVEEHKA TYSADTRFYP GQRLHHAVFG EGEVAAVEGN ASDPVIDVRF DQAGRRRLIA
RRAPIQLLEG SQSAILS
//
