ID A0A0D7PRT7_9BRAD Unreviewed; 361 AA.
AC A0A0D7PRT7;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN ORFNames=UP09_02025 {ECO:0000313|EMBL:KJC51691.1};
OS Bradyrhizobium sp. LTSP885.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=1619232 {ECO:0000313|EMBL:KJC51691.1, ECO:0000313|Proteomes:UP000032343};
RN [1] {ECO:0000313|Proteomes:UP000032343}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LTSP885 {ECO:0000313|Proteomes:UP000032343};
RX PubMed=25909973; DOI=10.1038/ismej.2015.54;
RA VanInsberghe D.S., Maas K.R., Cardenas E., Strachan C.R., Hallam S.J.,
RA Mohn W.;
RT "Non-symbiotic Bradyrhizobium ecotypes dominate North American forest
RT soils.";
RL ISME J. 9:2435-2441(2015).
CC -!- FUNCTION: Efflux system for nickel and cobalt.
CC {ECO:0000256|ARBA:ARBA00002510}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC {ECO:0000256|RuleBase:RU362101}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJC51691.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JYMT01000003; KJC51691.1; -; Genomic_DNA.
DR RefSeq; WP_044536340.1; NZ_JYMT01000003.1.
DR AlphaFoldDB; A0A0D7PRT7; -.
DR PATRIC; fig|1619232.5.peg.3001; -.
DR OrthoDB; 271709at2; -.
DR Proteomes; UP000032343; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR Pfam; PF03824; NicO; 2.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW Nickel {ECO:0000256|ARBA:ARBA00022596};
KW Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW Reference proteome {ECO:0000313|Proteomes:UP000032343};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU362101};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU362101};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT TRANSMEM 13..31
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 51..78
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 90..109
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 261..284
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 296..319
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 340..358
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
SQ SEQUENCE 361 AA; 39554 MW; 30F156FF50F0C68A CRC64;
MTGLSELLQQ GSAHAWLFVP SAILLGALHG MEPGHSKTMM AAFIIAVRGT VLQAALLGLS
AAISHTAIVW AVALGGLYFG RQWSGEQSEA YFQIASAVVI IGIAIWMAWR TWREQNAQDL
DQDHDHHHHH DEVIQFATGD GVLTLEIFED GVPPRWRVRA ERGLLPAPEA IVLDTLRPDG
VRQSFSFVSR DGFLESVDEV PEPHAFKARL SLRGRAEAFE TSFEEHDHDH SHMNLGEEDD
AHARAHAADI RKRFAGRTVT TWQIVLFGLS GGLIPCPAAI TVLLLCIQLK QFSLGFTLVV
CFGIGLAITM VSAGVLAALS LRHIERRWTG FSRFARQAPY ASAALIVLVG LYTGWLGFSA
I
//
