ID A0A0D8I9V3_9CLOT Unreviewed; 440 AA.
AC A0A0D8I9V3;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Fe-S oxidoreductase {ECO:0000313|EMBL:AKL96021.1};
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:KJF27043.1};
GN ORFNames=CACET_c25760 {ECO:0000313|EMBL:AKL96021.1}, TZ02_09555
GN {ECO:0000313|EMBL:KJF27043.1};
OS Clostridium aceticum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=84022 {ECO:0000313|EMBL:AKL96021.1, ECO:0000313|Proteomes:UP000035704};
RN [1] {ECO:0000313|EMBL:AKL96021.1, ECO:0000313|Proteomes:UP000035704}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1496 {ECO:0000313|EMBL:AKL96021.1,
RC ECO:0000313|Proteomes:UP000035704};
RA Poehlein A., Schiel-Bengelsdorf B., Gottschalk G., Duerre P., Daniel R.;
RT "Genome sequence of Clostridium aceticum DSM 1496.";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KJF27043.1, ECO:0000313|Proteomes:UP000032372}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1496 {ECO:0000313|EMBL:KJF27043.1,
RC ECO:0000313|Proteomes:UP000032372};
RA Song Y., Hwang S., Cho B.-K.;
RT "Draft genome sequence of Clostridium aceticum DSM 1496, a potential
RT butanol broducer through syngas fermentation.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP009687; AKL96021.1; -; Genomic_DNA.
DR EMBL; JYHU01000010; KJF27043.1; -; Genomic_DNA.
DR RefSeq; WP_044824719.1; NZ_JYHU01000010.1.
DR AlphaFoldDB; A0A0D8I9V3; -.
DR STRING; 84022.CACET_c25760; -.
DR KEGG; cace:CACET_c25760; -.
DR PATRIC; fig|84022.5.peg.140; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000032372; Unassembled WGS sequence.
DR Proteomes; UP000035704; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR025274; DUF4070.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13282; DUF4070; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000035704}.
FT DOMAIN 2..134
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 157..375
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 440 AA; 51640 MW; E905B47A7368B991 CRC64;
MKKTILLITP ENREINRFRR KQFNNFIQIT MPYLAAFIDE SKYEITLVDE YNQRIPYDRD
FSLVALTVNT SNATHCYEIA KIFKEKGSKI VMGGPHVTLL PDETEEHCDY IIIGEAEETW
PIFLNDFYHS TPSDRYSCSN IPSLNKLPIP RRDLIGKRYF TKGAVFATRG CPYKCSYCNL
KQIYCDEFRV RPVEDVIEDI RSIKSKYFVF WDDNFFGDIN YVKSLMKELK KLKKKWAAQV
TLERCKDEEL LQLAKESGCM YLFIGLESFS EENLQEVNKG INDVMKYKKI INAIHTYGIS
VQAGIIFGFD HDRKDTFEAT LKACNNLGID GTTVSILTPL PQTPLYQQLK KEERLLSKDW
RHYNGKTSVV YKPKNMTKEE LYEGYMWFRR EFYSFKSILK RMIISKTNIM HNLIVNLGYK
LSIRENGFKK ANKDIETKHN
//
