ID   A0A0D9WYB5_9ORYZ        Unreviewed;       881 AA.
AC   A0A0D9WYB5;
DT   27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT   27-MAY-2015, sequence version 1.
DT   24-JAN-2024, entry version 26.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS   Leersia perrieri.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Leersia.
OX   NCBI_TaxID=77586 {ECO:0000313|EnsemblPlants:LPERR07G10600.1, ECO:0000313|Proteomes:UP000032180};
RN   [1] {ECO:0000313|EnsemblPlants:LPERR07G10600.1, ECO:0000313|Proteomes:UP000032180}
RP   NUCLEOTIDE SEQUENCE.
RA   Wing R.A.;
RT   "Oryza genome evolution.";
RL   Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:LPERR07G10600.1, ECO:0000313|Proteomes:UP000032180}
RP   NUCLEOTIDE SEQUENCE.
RA   Yu Y., Lee S., de Baynast K., Wissotski M., Liu L., Talag J.,
RA   Goicoechea J., Angelova A., Jetty R., Kudrna D., Golser W., Rivera L.,
RA   Zhang J., Wing R.;
RL   Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EnsemblPlants:LPERR07G10600.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (APR-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
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DR   AlphaFoldDB; A0A0D9WYB5; -.
DR   STRING; 77586.A0A0D9WYB5; -.
DR   EnsemblPlants; LPERR07G10600.1; LPERR07G10600.1; LPERR07G10600.
DR   Gramene; LPERR07G10600.1; LPERR07G10600.1; LPERR07G10600.
DR   eggNOG; KOG2119; Eukaryota.
DR   Proteomes; UP000032180; Chromosome 7.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF21; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 2.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 2.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000032180}.
FT   DOMAIN          102..159
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          191..372
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          463..824
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
SQ   SEQUENCE   881 AA;  99695 MW;  692AC834292B372C CRC64;
     MVSGNMFYRR KRSWRANDLV SRSTLQLLDF DDGSPPEYAW RRKLSSHANR LKEFNVTFRE
     AFKMMKLGLR LWSYIREEAS HGRKAPIDPF TRESDRPSAS QGVPLGGMGC VLSDGCFTHL
     SRSGSISRGF RGEFKHWQII PGSCEMSAVM ANQFSVTRNP AMMVYHPGIG NYEVIVLHTM
     HYFLERGLSM MIVNTGKERA KVSLVMTWAV NSIGGLSHHS GEENMEFLAC FYITSKTANG
     NPPVTFAVAA CENQNVNVTV LPAFGLSGES SITAREMWGT LLQDGCFDRD NFSAGPSMPS
     SLGDTVCAAV CASTWVEPNG RCTVVFSLAW SSPKIKFKKG NEYYRRYTKF YGTSPRSAIN
     LVQDSLMKYK HWEEEIDKWQ SPILHDERLP EWYKVILFNE LYFLVAGGTV WIDSESLMDD
     TNEMLNSRLP EDSDLPLCDS TRKSAVPLIG LTPHIIDDRE NVGKFLYLEG IEYFMWCTYD
     VHFYASFALL ELFPKIELSI QRDFATAVLR EDRSRVRFLA DGTWGTRKVI GAVAHDLGAH
     DPWHELNAYN IHDTSRWKDL NPKFVLQVYR DFAATCDMSF GKDVWPAVCT AMEYMEQFDH
     DGDGMIENDG FPDQTYDAWT VRGVSAYCGC LWLAALQAAA ALARSLGHHD YAERCMVKFA
     KAKPVFEAKL WNGSYFNYDS GTSYNSRSIH ADQLAGQWYT ASSGLPPLFD EDRIKCTLQK
     IFDYNVMRVK GGRMGAVNGM HPNGKVDETC MQSREIWTGV TYSLAATMLL HGMDSQAFTT
     AEGIFIAGWS EEGYGYWFQT PEAWTIDGHY RSLIYMRPLA IWAMQYALSP PRSILEAPKV
     NTMDRTSYIS PSTLQFLQDS VRKMTPKNSC FGSNSLNCDE C
//