ID A0A0E0ESE7_9ORYZ Unreviewed; 527 AA.
AC A0A0E0ESE7;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=5'-nucleotidase {ECO:0008006|Google:ProtNLM};
OS Oryza meridionalis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=40149 {ECO:0000313|EnsemblPlants:OMERI09G08560.2};
RN [1] {ECO:0000313|EnsemblPlants:OMERI09G08560.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=OR44 {ECO:0000313|EnsemblPlants:OMERI09G08560.2};
RA Wing R.A., Panaud O., Henry R.;
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblPlants:OMERI09G08560.2}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (APR-2015) to UniProtKB.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
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DR AlphaFoldDB; A0A0E0ESE7; -.
DR EnsemblPlants; OMERI09G08560.2; OMERI09G08560.2; OMERI09G08560.
DR Gramene; OMERI09G08560.2; OMERI09G08560.2; OMERI09G08560.
DR Proteomes; UP000008021; Chromosome 9.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07522; HAD_cN-II; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF12; 5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 1; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000008021}.
FT ACT_SITE 98
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 100
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 98
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 100
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 372
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 527 AA; 60616 MW; 5CDABB5797A151E2 CRC64;
MAAARLRLLS AGLLSPAVSR ARHSSSLQGL RALSTLSTTL GSGAGEDEIE RIRREFEDAK
RNYLSIPVAI KDMAKMNPQG IYVNKNVKLD DLQVYGFDYD YTLSHYSEHL QCLIYDLAKK
HLVNELKYPE SCLKYEYDHG FPIRGLYYDR LKGCLLKLDF FGSIEPDGCF FGRRKLSLSE
IKELYGTRHI GRDQARQLVG LMDVFCFSEH FVDAKLEFDA SYVYEDVNQS IQHVHRSGLI
HRKILSEPQK YLIKNSQVFR FLKMLREKGK NLFLLTNSPF YFVDGGMSYL LEDEHFDGNS
WRELFDVVIA QANKPTFYNS DHPFRVYDTQ KDTLAFTAVD KFLPNEVYYH GCLKSFLQIT
KWRGPEVIYF GDHLLSDLRG PAKAGWRTAA VIRELEDEIE IQNGDSYRFQ QAKLSIIHDL
LGKVHATVVS TEKGQVYRAL LDELNAERRQ CRSAMRDLFN SSFGATFLTD TGRESSFAYH
IHQYADIYTS KLENFLSYAP ESWLHPPHDI KIMPHNAKVP ASLFSGS
//
