ID   A0A0E0HZU6_ORYNI        Unreviewed;       849 AA.
AC   A0A0E0HZU6;
DT   27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT   27-MAY-2015, sequence version 1.
DT   28-JUN-2023, entry version 27.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS   Oryza nivara (Indian wild rice) (Oryza sativa f. spontanea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=4536 {ECO:0000313|EnsemblPlants:ONIVA07G10450.2};
RN   [1] {ECO:0000313|EnsemblPlants:ONIVA07G10450.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=SL10 {ECO:0000313|EnsemblPlants:ONIVA07G10450.2};
RA   Wing R.A., Hsing Y.;
RL   Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:ONIVA07G10450.2}
RP   IDENTIFICATION.
RC   STRAIN=SL10 {ECO:0000313|EnsemblPlants:ONIVA07G10450.2};
RG   EnsemblPlants;
RL   Submitted (APR-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
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DR   AlphaFoldDB; A0A0E0HZU6; -.
DR   EnsemblPlants; ONIVA07G10450.2; ONIVA07G10450.2; ONIVA07G10450.
DR   Gramene; ONIVA07G10450.2; ONIVA07G10450.2; ONIVA07G10450.
DR   Proteomes; UP000006591; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF21; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 2.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 3.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006591}.
FT   DOMAIN          102..252
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          265..398
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          476..793
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
FT   REGION          85..107
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   849 AA;  95640 MW;  4A7055DF9937DDA8 CRC64;
     MVSSNMFYRR KRSWRAGDLV SRSTLQLLDF DDGSPPEYAW RRKLSSHANR LKEFNVTFRE
     AIKMMKLGLR LWSYIREEAS HGRKAPIDPF TRESDKPSAS QGVPLGGMGS GSISRGFRGE
     FKHWQIIPGS CEMSPVMANQ FSIFITRGGN KKYSSVLAPG QHDSLKKSND DGISSWDWKL
     RGDRSTYHAL FPRAWTVYDG EPDPELKVSC RQISPFIPHN YQESSLPTSV FVYTIVNTGK
     ERAKVSLVMT WAVGFPIIPA VMTANNNPPV TFAVAACENQ NVNVTVLPAF GLSGESSVTA
     REMWGTLSQD GCFDRDNFSA GPSMPSLLGD TVCAAVCAST WVEPHGRCTV VFSLAWSSPK
     VKFKKGNAYY RRYTKFYGTS PRSAINLVQD SLMKYKHWEE EIDKWQRPIL HDERFPEWYK
     VTLFNELYFL VAGGTVWIDS ASLMADADEM MNSRLSEDND LPLHHSSRNS AVPLIAVLRE
     DKSRVRFLAD GTWGTRKVIG AVAHDLGAHD PWHELNAYNI HDTSRWKDLN PKFVLQVYRD
     FAATGDMSFG KDVWPAVCTA MEYMEQFDHD DDGMIENDGF PDQTYDAWTV RGVSAYCGCL
     WLAALQAAAA LARTLGHHDY AERCMLKFAK AKPVFEAKLW NGSYFNYDSG TSYNSRSIHA
     DQLAGQWYTA SSGLPPLFDE GRIKCTLQKI FDYNVMRVKG GRMGAVNGMH PNGKVDETCM
     QSREIWTGVT YSLAATMLLH GMDDQAFTTA EGIFTAGWSE EGYGYWFQTP EAWTIDGHYR
     SLIYMRPLAI WAMQYALSPP RTILEAPKVN TMDRTSYISP STLQFLQDSV RKMTPKNSCF
     GSSSSNCEC
//