ID   A0A0E0J055_ORYNI        Unreviewed;       914 AA.
AC   A0A0E0J055;
DT   27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT   27-MAY-2015, sequence version 1.
DT   24-JAN-2024, entry version 39.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS   Oryza nivara (Indian wild rice) (Oryza sativa f. spontanea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=4536 {ECO:0000313|EnsemblPlants:ONIVA11G08140.1};
RN   [1] {ECO:0000313|EnsemblPlants:ONIVA11G08140.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=SL10 {ECO:0000313|EnsemblPlants:ONIVA11G08140.1};
RA   Wing R.A., Hsing Y.;
RL   Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:ONIVA11G08140.1}
RP   IDENTIFICATION.
RC   STRAIN=SL10 {ECO:0000313|EnsemblPlants:ONIVA11G08140.1};
RG   EnsemblPlants;
RL   Submitted (APR-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
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DR   AlphaFoldDB; A0A0E0J055; -.
DR   STRING; 4536.A0A0E0J055; -.
DR   EnsemblPlants; ONIVA11G08140.1; ONIVA11G08140.1; ONIVA11G08140.
DR   Gramene; ONIVA11G08140.1; ONIVA11G08140.1; ONIVA11G08140.
DR   eggNOG; KOG2119; Eukaryota.
DR   HOGENOM; CLU_006322_1_0_1; -.
DR   OMA; HDLGAPN; -.
DR   Proteomes; UP000006591; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF3; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 2.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 2.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006591};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT   DOMAIN          97..229
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          239..384
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          499..854
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   914 AA;  102821 MW;  38BF03D38690603D CRC64;
     MVENGVLEQP KGVSRNRPRA QSNDHPVDPG YLPELTWEHK LSNIGYDLPS FRLTWRETFQ
     LAGLGLRLGR HILEETSKGR AAVIDPMKKR IAKSGQGVPL GGIGSGSIGR SYKGEFQRWQ
     LFPGTCEERP VLANQFSAFI SRKDGRNYSS VLHPGKPDLP KGSNISGIGS WDWNMSGQNS
     TYHALYPRSW TIYNGEPDPD VNIVCRQISP IIPHNYQQSS YPVSVFTFTN SVGGKSELTG
     YHSNSPMITA NGQPPVTFAI AAQEKEDVHI SECPYFIISG SSDAFSAKDM WNYVKENGSF
     DNLDLTKTSM CSKPGLSIGA AIAASVKLPP QTTQNVSFAL AWACPEVKFS SGKTYHRRYT
     KFHGTDNDAA ASLAHDAILE HNSWERQIEE WQNPILQDER FPDWYPVTLF NELYYLNAGG
     TIWTDGLPPI QSLTGIGEKK FSLDMQNGDA DDANGIIPRN NTASDILNQM ASVLERIHAS
     MESNSAIGTT LLQGEENIGQ FLYLEGIEYY MWNTYDVHFY ASFSLIMLFP KLQLSIQRDF
     AAAVLMHDPE KLRMLHDGKW VARKVLGAIP HDLGLYDPWF KVNAYTLYNT DRWKDLNPKF
     VLQVYRDVVA TGDKSFARAV WPSVYMAMAY MEQFDRDKDG MIENEDFPDQ TYDVWSMAGI
     SAYCGGLWVA ALQAASALAH EVGDKASEKL FWDKYEKAKS VYGKLWNGSY FNYDDGDNIM
     SASIHADQLA GQWYAKACGL FPIVDKDKAE SALEKIYSFN DGKRGAMNGM WPDGTVDMSA
     MQSREIWPGV TYALAATMIQ EGMVEKGFKT AEGIYHAAWS PEGLGYSFQT PEAWNNDDEY
     RSLCYMRPLA IWAIQWALSN PKLHKQTADI PQDSFPKNQF SYARIAKLLH LPEDESPKSF
     LRVIYEIVRN RYRS
//