ID A0A0E0NCS0_ORYRU Unreviewed; 647 AA.
AC A0A0E0NCS0;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
OS Oryza rufipogon (Brownbeard rice) (Asian wild rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=4529 {ECO:0000313|EnsemblPlants:ORUFI02G11600.4, ECO:0000313|Proteomes:UP000008022};
RN [1] {ECO:0000313|Proteomes:UP000008022}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. W1943 {ECO:0000313|Proteomes:UP000008022};
RA Zhao Q.;
RL Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblPlants:ORUFI02G11600.4}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; A0A0E0NCS0; -.
DR EnsemblPlants; ORUFI02G11600.2; ORUFI02G11600.2; ORUFI02G11600.
DR EnsemblPlants; ORUFI02G11600.4; ORUFI02G11600.4; ORUFI02G11600.
DR Gramene; ORUFI02G11600.2; ORUFI02G11600.2; ORUFI02G11600.
DR Gramene; ORUFI02G11600.4; ORUFI02G11600.4; ORUFI02G11600.
DR Proteomes; UP000008022; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR NCBIfam; TIGR02432; lysidine_TilS_N; 1.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Reference proteome {ECO:0000313|Proteomes:UP000008022}.
FT DOMAIN 71..248
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
SQ SEQUENCE 647 AA; 71743 MW; DDBF7A84B1723D7D CRC64;
MMPLLLLLLP VRSAPLRRLL LCRCSSSSSS AAAASASSSA CPVPASSVLA PYHRAFARRM
ALAGVHPHHR VAVGVSGGPD SMALCVLAAA WKKAGEGREQ EDEGEGGVSG FVDGLLGVVV
DHGLRPESAD EAQLVRVVCE IATCEWPNGR PKLGHIQEAA REMRYQKLLD ICIKQRIAVL
LIAHHSDDQA ELFVLRLSRN SGVLGLAGTA FVSQLFAPNL KYDGDNFSRY GVILVRPMLE
FSKDDMYKSE LHKLIYACRL TRAFIDNACS MVLKKSLTIM EHGYAVIDLE KLDPHNVDDL
FLSRYLAYVL QFVSQRHRPL RGRSAQLLID YIRTIPCKAA LSVAGCYLCA VPRSKGTKVL
VCCSVDLMES SSVHMSYKCS YVKQPPPVSE INQIVTEARI YSDQFRQNCP NTPFPSSKFS
TDVLNKAKDL KLIGDCTLEK LNYLQTDEHQ KFITTKEHGQ EQYLEKTSFP YLEVLNLWPG
ETCHFMGRFL ITWRTSEVVV NGMCLHDSQK HTCQYCMVNQ DGSLAIRHMF DTDWLFLAEV
CKIHSLEENK NYSNALCDKL EDAKLVQHSR YLQLSAMKSL QILRSIPAPA RRMLPVLTNS
QGAVLSIPCQ PGVAEAIATF GTRQVEMKVP LDHCESNFVS AGGGSSA
//
