ID   A0A0E0QDI7_ORYRU        Unreviewed;       938 AA.
AC   A0A0E0QDI7;
DT   27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT   27-MAY-2015, sequence version 1.
DT   24-JAN-2024, entry version 31.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
OS   Oryza rufipogon (Brownbeard rice) (Asian wild rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX   NCBI_TaxID=4529 {ECO:0000313|EnsemblPlants:ORUFI08G00880.1, ECO:0000313|Proteomes:UP000008022};
RN   [1] {ECO:0000313|Proteomes:UP000008022}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. W1943 {ECO:0000313|Proteomes:UP000008022};
RA   Zhao Q.;
RL   Submitted (JUN-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:ORUFI08G00880.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC         acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC         ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC         ChEBI:CHEBI:52639; EC=3.2.1.45;
CC         Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
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DR   AlphaFoldDB; A0A0E0QDI7; -.
DR   STRING; 4529.A0A0E0QDI7; -.
DR   EnsemblPlants; ORUFI08G00880.1; ORUFI08G00880.1; ORUFI08G00880.
DR   Gramene; ORUFI08G00880.1; ORUFI08G00880.1; ORUFI08G00880.
DR   eggNOG; KOG2119; Eukaryota.
DR   HOGENOM; CLU_006322_1_0_1; -.
DR   OMA; TIWTDGQ; -.
DR   Proteomes; UP000008022; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR12654:SF27; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008022}.
FT   DOMAIN          85..407
FT                   /note="Glycosyl-hydrolase family 116 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF12215"
FT   DOMAIN          513..873
FT                   /note="Glycosyl-hydrolase family 116 catalytic region"
FT                   /evidence="ECO:0000259|Pfam:PF04685"
SQ   SEQUENCE   938 AA;  102536 MW;  1AA89B261E6D4508 CRC64;
     MGSSELEETK AVLHMDCEKP PAISWERTFD DEGKKVAMFS MTLNDMMAIV PLMIKMLGLN
     LKDNAKGLAS VYDPLKKWMD NCYRGVPLGG IGAGSIGRSY RGYFQQFQIF PSIYEEKPIL
     ANQFSAFISR PDGKRYSTVL SAPNADVLKG IDKAGIGSWD WKLKEKNCTY HGLFPRSWTV
     YNGEPDPEIK ITCRQISPFI PHNYKESSFP VAVFTFTLHN SGSTPADVTL LFTWANSVGG
     KSELTGNHKN SRMTARDGVH GVLLHHRTAD GRPPVTFAIA SRETDGVRVT GCPRFTMGPS
     NSGDVTAKDM WDQINKNGSF VGDGNAAAAA TGASRPGSSI GAAVAATTTV AAGGARAVSF
     ALSWSCPEVK FPAGRTYHRR YTKFHGTDRD AAAERLAHDA LLEHMKWESQ IEEWQRPILQ
     DKSLPEWYPI TLFNELYYLN AGGTIWTDGQ PPKNTSLSSA TEPFNLDTFS TVANGGSAVD
     GILSTVAAAA ARSNTAAAAM GTALLRDGEE NVGQVLYLEG MEYNMWNTYD VHFYASFALL
     SLFPELELNL QRDFVRGVLL HDPCLRRTLD GATVARKVLG AVPHDMGLND PWFEVNAYML
     HDPVRWKDLN PKFVLQVYRD VVATGNAGFA EAAWPAVYLA MAYMDQFDRD GDGMVENEGR
     PDQTYDLWSV SGVSAYTGGL WVAALQAAAA MAGIVGDGAA EAYFRGRYHR ARRVYTDELW
     NGGYFNYDNS GGATSSSIQA DQLAGQWYAR ACGLEPIVDG DKARRALATV LDYNVMRVKG
     GAIGAVNGMR PDGAVDASST QSKEVWPGVT YAVAAAMIHE GMPEAAFKTA KGIHDAGWGK
     HGFGYAFQTP ESWTADGGYR ALHYMRPLGV WAMQWALSPP VLHKEHRVAA VAASPEDAAL
     GQEKFDKVAS MLRLPEEQQH KGILRALYDT LRQLLLPS
//