ID A0A0E3SGC9_METBA Unreviewed; 526 AA.
AC A0A0E3SGC9;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|HAMAP-Rule:MF_01904};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_01904};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_01904};
DE EC=4.1.1.31 {ECO:0000256|HAMAP-Rule:MF_01904};
GN Name=ppcA {ECO:0000256|HAMAP-Rule:MF_01904};
GN ORFNames=MSBR3_0916 {ECO:0000313|EMBL:AKB81494.1};
OS Methanosarcina barkeri 3.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=1434107 {ECO:0000313|EMBL:AKB81494.1, ECO:0000313|Proteomes:UP000033066};
RN [1] {ECO:0000313|EMBL:AKB81494.1, ECO:0000313|Proteomes:UP000033066}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3 {ECO:0000313|EMBL:AKB81494.1,
RC ECO:0000313|Proteomes:UP000033066};
RA Henriksen J.R., Luke J., Reinhart S., Benedict M.N., Youngblut N.D.,
RA Metcalf M.E., Whitaker R.J., Metcalf W.W.;
RT "Methanogenic archaea and the global carbon cycle.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC dicarboxylic acid source for the tricarboxylic acid cycle.
CC {ECO:0000256|HAMAP-Rule:MF_01904}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01904}.
CC -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000256|HAMAP-
CC Rule:MF_01904}.
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DR EMBL; CP009517; AKB81494.1; -; Genomic_DNA.
DR RefSeq; WP_048106842.1; NZ_CP009517.1.
DR AlphaFoldDB; A0A0E3SGC9; -.
DR STRING; 1434107.MSBR3_0916; -.
DR GeneID; 24788409; -.
DR KEGG; mbak:MSBR3_0916; -.
DR PATRIC; fig|1434107.4.peg.1207; -.
DR HOGENOM; CLU_517433_0_0_2; -.
DR OrthoDB; 85849at2157; -.
DR Proteomes; UP000033066; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_01904; PEPcase_type2; 1.
DR InterPro; IPR007566; PEP_COase_arc-type.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR NCBIfam; TIGR02751; PEPCase_arch; 1.
DR Pfam; PF14010; PEPcase_2; 1.
DR PIRSF; PIRSF006677; UCP006677; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|HAMAP-Rule:MF_01904};
KW Lyase {ECO:0000256|HAMAP-Rule:MF_01904, ECO:0000313|EMBL:AKB81494.1};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_01904};
KW Pyruvate {ECO:0000313|EMBL:AKB81494.1}.
SQ SEQUENCE 526 AA; 58862 MW; 73A9AB8B2D160271 CRC64;
MNRKTVFPKV MCTQHPDSAS RYISTQEEPE EAIEAAQVFG CDEYMPDYEG KATPYHQNVQ
IVSKFIEETD LIPGKDIFIT PRAPSAVQEN RFRQLMVMMS IAEANYNALE YSDIQAINEF
VHPMTDSVRE IIEAQQHMVD VSELAKKEFG FSMEVPRIIP LIEDAPALLH ARELAENTLL
AWKGHFGTDP EKFRVFLGKS DSALSFGHVA STLSCKYAIN GICELNSELD TQTGIIFGAG
TLPFRGHLDL KNAENFFREY RGVGTITLQS ALRYSHKKGD AESLVNLAKA RLPETPEFFS
VEEKEEIINL IGIFGTSYSR ILRQLAPVIN RVADLLPQQR DRLMHKGTGG YSRSAPDISG
LVKFCRTDIA KELEASMPAE DLHLPRAIKF TGALYSIGLP PEFIGTGRAL EEARKKLGEA
ACENLLTKYF PSLEGDLNFA SGYLDLNVAS RFLSRDCLKE VRKDLDILHE IFDLETSPEP
SYRILVEMMQ PDLLQAGSRG NCMDEEVSQL VCSTLTKMGK IRKALG
//
