ID A0A0E3XQA1_MYCCH Unreviewed; 199 AA.
AC A0A0E3XQA1;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=3-isopropylmalate dehydratase small subunit {ECO:0000256|HAMAP-Rule:MF_01031};
DE EC=4.2.1.33 {ECO:0000256|HAMAP-Rule:MF_01031};
DE AltName: Full=Alpha-IPM isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
DE Short=IPMI {ECO:0000256|HAMAP-Rule:MF_01031};
DE AltName: Full=Isopropylmalate isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
GN Name=leuD {ECO:0000256|HAMAP-Rule:MF_01031,
GN ECO:0000313|EMBL:MBF9522357.1};
GN ORFNames=BKG82_23455 {ECO:0000313|EMBL:OHU49678.1}, BKG84_19735
GN {ECO:0000313|EMBL:OHU80290.1}, GR01_15750
GN {ECO:0000313|EMBL:AKC39714.1}, HA136_15540
GN {ECO:0000313|EMBL:MBF9522357.1};
OS Mycobacteroides chelonae (Mycobacterium chelonae).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacteroides.
OX NCBI_TaxID=1774 {ECO:0000313|EMBL:AKC39714.1, ECO:0000313|Proteomes:UP000033056};
RN [1] {ECO:0000313|EMBL:AKC39714.1, ECO:0000313|Proteomes:UP000033056}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35752 {ECO:0000313|EMBL:AKC39714.1,
RC ECO:0000313|Proteomes:UP000033056};
RX PubMed=26021923;
RA Hasan N.A., Davidson R.M., de Moura V.C., Garcia B.J., Reynolds P.R.,
RA Epperson L.E., Farias-Hesson E., DeGroote M.A., Jackson M., Strong M.;
RT "Draft Genome Sequence of Mycobacterium chelonae Type Strain ATCC 35752.";
RL Genome Announc. 3:e00536-15(2015).
RN [2] {ECO:0000313|Proteomes:UP000179441, ECO:0000313|Proteomes:UP000180043}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=15515 {ECO:0000313|EMBL:OHU49678.1,
RC ECO:0000313|Proteomes:UP000180043}, and 15518
RC {ECO:0000313|EMBL:OHU80290.1, ECO:0000313|Proteomes:UP000179441};
RA Fogelson S.B., Camus A.C., Lorenz W., Vasireddy R., Vasireddy S., Smith T.,
RA Brown-Elliott B.A., Wallace R.J.Jr., Hasan N.A., Reischl U., Sanchez S.;
RT "Evaluation of Human, Veterinary and Environmental Mycobacterium chelonae
RT Isolates by Core Genome Phylogenomic Analysis, Targeted Gene Comparison,
RT and Anti-microbial Susceptibility Patterns: A Tale of Mistaken
RT Identities.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:MBF9522357.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Myco6 {ECO:0000313|EMBL:MBF9522357.1};
RX PubMed=33166284;
RA Gu C.H., Zhao C., Hofstaedter C., Tebas P., Glaser L., Baldassano R.,
RA Bittinger K., Mattei L.M., Bushman F.D.;
RT "Investigating hospital Mycobacterium chelonae infection using whole genome
RT sequencing and hybrid assembly.";
RL PLoS ONE 15:0-e0236533(2020).
RN [4] {ECO:0000313|EMBL:MBF9522357.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Myco6 {ECO:0000313|EMBL:MBF9522357.1};
RA Gu C.H., Zhao C., Bushman F.D.;
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000256|ARBA:ARBA00002695, ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33; Evidence={ECO:0000256|ARBA:ARBA00000491,
CC ECO:0000256|HAMAP-Rule:MF_01031};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4. {ECO:0000256|ARBA:ARBA00004729,
CC ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000256|ARBA:ARBA00011271,
CC ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009845, ECO:0000256|HAMAP-Rule:MF_01031}.
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DR EMBL; CP010946; AKC39714.1; -; Genomic_DNA.
DR EMBL; JAAOOS010000002; MBF9522357.1; -; Genomic_DNA.
DR EMBL; MLIQ01000024; OHU49678.1; -; Genomic_DNA.
DR EMBL; MLIS01000001; OHU80290.1; -; Genomic_DNA.
DR RefSeq; WP_046254246.1; NZ_MLIU01000016.1.
DR GeneID; 31680794; -.
DR PATRIC; fig|1774.35.peg.3196; -.
DR HOGENOM; CLU_081378_0_1_11; -.
DR OrthoDB; 9777465at2; -.
DR UniPathway; UPA00048; UER00071.
DR Proteomes; UP000033056; Chromosome.
DR Proteomes; UP000179441; Unassembled WGS sequence.
DR Proteomes; UP000180043; Unassembled WGS sequence.
DR Proteomes; UP000699007; Unassembled WGS sequence.
DR GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR HAMAP; MF_01031; LeuD_type1; 1.
DR InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR NCBIfam; TIGR00171; leuD; 1.
DR PANTHER; PTHR43345:SF5; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR43345; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605, ECO:0000256|HAMAP-
KW Rule:MF_01031};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304,
KW ECO:0000256|HAMAP-Rule:MF_01031}; Isomerase {ECO:0000313|EMBL:AKC39714.1};
KW Leucine biosynthesis {ECO:0000256|ARBA:ARBA00022430, ECO:0000256|HAMAP-
KW Rule:MF_01031};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01031}.
FT DOMAIN 1..111
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 199 AA; 22114 MW; 8B25F58E7FE31F7B CRC64;
MEAFDTHTGI GVPLRRSNVD TDQIIPAVYL KRVTRTGFED GLFAGWRADP SFILNQEPFN
RGSVLVAGPD FGTGSSREHA VWALMDYGFR VVLSSRFGDI FRGNAGKAGL VAGLVDQSNI
ELLWKLIEQN PGLELTVNLD DRTVTAGTAV VPFEIDDYTR WRLLEGLDDI GLTLRKVDEI
EAFEASRPEF KPRTLEPSE
//
