ID A0A0E9MRX1_9SPHN Unreviewed; 484 AA.
AC A0A0E9MRX1;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN ORFNames=SCH01S_46_00220 {ECO:0000313|EMBL:GAO40314.1};
OS Sphingomonas changbaiensis NBRC 104936.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1219043 {ECO:0000313|EMBL:GAO40314.1, ECO:0000313|Proteomes:UP000033202};
RN [1] {ECO:0000313|EMBL:GAO40314.1, ECO:0000313|Proteomes:UP000033202}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 104936 {ECO:0000313|EMBL:GAO40314.1,
RC ECO:0000313|Proteomes:UP000033202};
RA Katano-Makiyama Y., Hosoyama A., Hashimoto M., Noguchi M., Tsuchikane K.,
RA Ohji S., Yamazoe A., Ichikawa N., Kimura A., Fujita N.;
RT "Whole genome shotgun sequence of Sphingomonas changbaiensis NBRC 104936.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAO40314.1}.
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DR EMBL; BBWU01000046; GAO40314.1; -; Genomic_DNA.
DR RefSeq; WP_046349114.1; NZ_BBWU01000046.1.
DR AlphaFoldDB; A0A0E9MRX1; -.
DR STRING; 1219043.SCH01S_46_00220; -.
DR OrthoDB; 7440981at2; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000033202; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000033202};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..275
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 316..467
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 484 AA; 52012 MW; B54FFC452904A989 CRC64;
MQQLSGQDAS FVYLETPHTP MHIGSVGIYD PSTAPGGFVR FKDILDFVGS RLGGARSFRQ
RLVRLPLDLD HPYWIEDPAF DIEFHIRHIA LPAPGDWRQL CIQVARLHSR PLDLSKPLWE
FTIIEGLDNI EGLPKGCFAL LAKVHHAAID GMSGVELSAA IHDLSPDKPN RIANDDWAAD
DLPGVADLLV KSYFNALRKP LQYAETMARA VPGLARLSQS VSKGDVSLAN QKLAPKTRLN
GKVGPHRVFD AVPMPLADIR AIKDAVPGAT VNDVILAIVG GGLRHYLQGL GELPAETMTA
MAPISVRAEG EKAALGNLVS AMVVGLGTHI GDPIERLRYV HGEAVNSKAL TNAVGARTLT
DFSQFMPAGL SGIAARLYTR LGAANAHAPV FNTVVTNVPG SRVPLYFAGA RMVAMYGLAP
VFDSMGLINT IYSYVDTIAI SFTADREMMP DPAAYADGLR KAYEELKAAA VPAAKPKKGA
KQHA
//
