ID   A0A0E9N226_9BACT        Unreviewed;       317 AA.
AC   A0A0E9N226;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   SubName: Full=Endo-1,5-alpha-L-arabinanase {ECO:0000313|EMBL:GAO43869.1};
GN   Name=abnA {ECO:0000313|EMBL:GAO43869.1};
GN   ORFNames=FPE01S_02_09750 {ECO:0000313|EMBL:GAO43869.1};
OS   Flavihumibacter petaseus NBRC 106054.
OC   Bacteria; Bacteroidota; Chitinophagia; Chitinophagales; Chitinophagaceae;
OC   Flavihumibacter.
OX   NCBI_TaxID=1220578 {ECO:0000313|EMBL:GAO43869.1, ECO:0000313|Proteomes:UP000033121};
RN   [1] {ECO:0000313|EMBL:GAO43869.1, ECO:0000313|Proteomes:UP000033121}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 106054 {ECO:0000313|EMBL:GAO43869.1,
RC   ECO:0000313|Proteomes:UP000033121};
RA   Miyazawa S., Hosoyama A., Hashimoto M., Noguchi M., Tsuchikane K., Ohji S.,
RA   Yamazoe A., Ichikawa N., Kimura A., Fujita N.;
RT   "Whole genome shotgun sequence of Flavihumibacter petaseus NBRC 106054.";
RL   Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC       {ECO:0000256|ARBA:ARBA00004834, ECO:0000256|PIRNR:PIRNR026534}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|PIRNR:PIRNR026534}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GAO43869.1}.
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DR   EMBL; BBWV01000002; GAO43869.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0E9N226; -.
DR   STRING; 1220578.FPE01S_02_09750; -.
DR   OrthoDB; 9801455at2; -.
DR   UniPathway; UPA00667; -.
DR   Proteomes; UP000033121; Unassembled WGS sequence.
DR   GO; GO:0046558; F:arabinan endo-1,5-alpha-L-arabinosidase activity; IEA:InterPro.
DR   GO; GO:0031222; P:arabinan catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd18830; GH43_CjArb43A-like; 1.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR016840; Glyco_hydro_43_endo_a_Ara-ase.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR   PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   PIRSF; PIRSF026534; Endo_alpha-L-arabinosidase; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR026534};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR026534};
KW   Reference proteome {ECO:0000313|Proteomes:UP000033121}.
FT   ACT_SITE        20
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-1"
FT   ACT_SITE        203
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-1"
FT   BINDING         20
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT   BINDING         97
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT   BINDING         137..140
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT   BINDING         157..159
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT   SITE            140
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-3"
FT   SITE            273
FT                   /note="Important for substrate recognition"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR026534-3"
SQ   SEQUENCE   317 AA;  35642 MW;  2F2019E4B6FD8DCD CRC64;
     MIGLLPVAGY ATDSLPGIHD PVIIREKGYW YVFGTGRGIT VMRSADRRNW KRLPPVFDQP
     PGWAVKAVSG FKGHIWAPDI AYVRGHYYLF YAVSAFGKNT SCIGVASNKT LDPDSKEYHW
     LDHGKVIESV PGRDMWNAID PNLILDEKGT PWLSFGSFWN GIKMVQLDSS LTQPATPQQW
     YTLAARSRNP VLPDSVAGDA AIEAPFIFHH NNYYYLFVSF DYCCRGVKST YKVVVGRSKS
     IIGPYLDRDG VPMTLGGGTL VREGDANWMG IGHNAVFSDD GNDYLLYHGY DAHDNGKSKL
     WQEKIRWRDD WPEAVIK
//