ID A0A0F0KNC1_9MICO Unreviewed; 522 AA.
AC A0A0F0KNC1;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE SubName: Full=Beta-xylosidase {ECO:0000313|EMBL:KJL21954.1};
DE EC=3.2.1.37 {ECO:0000313|EMBL:KJL21954.1};
GN Name=xynB_1 {ECO:0000313|EMBL:KJL21954.1};
GN ORFNames=RL72_02261 {ECO:0000313|EMBL:KJL21954.1};
OS Microbacterium azadirachtae.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=582680 {ECO:0000313|EMBL:KJL21954.1, ECO:0000313|Proteomes:UP000033448};
RN [1] {ECO:0000313|EMBL:KJL21954.1, ECO:0000313|Proteomes:UP000033448}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 23848 {ECO:0000313|EMBL:KJL21954.1,
RC ECO:0000313|Proteomes:UP000033448};
RA Corretto E.;
RT "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT metal contaminated environments.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJL21954.1}.
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DR EMBL; JYIT01000079; KJL21954.1; -; Genomic_DNA.
DR RefSeq; WP_045250941.1; NZ_JYIT01000079.1.
DR AlphaFoldDB; A0A0F0KNC1; -.
DR PATRIC; fig|582680.7.peg.2309; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000033448; Unassembled WGS sequence.
DR GO; GO:0009044; F:xylan 1,4-beta-xylosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000033448}.
FT DOMAIN 336..512
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 187
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 126
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 522 AA; 56272 MW; A4C43E82B7A653E5 CRC64;
MIAAANPILP GCHPDPSICR VGEWYYLVTS TFEYLPGLPV LRSRDLIRWE TVAHVIDRPG
MLDFDGIGSS GGLYAPTIRH GLGLFWVVCT LVDRDDGSRG GNFLVTASDP AGPWSDPVML
DAAGIDPSIA FDDEGRVWLH GTRLATAPEW HDQTEVWVRE YAPAERRLVG AEHVIWRGAV
RGAVWAEGPH LYRVDGRWLL IAAEGGTEFH HAVSAARADE IAGPYAGDRA NPVFTHRQLG
RTSPVIGAGH ADLVQAADGS WFAVMLAMRA DDGRHYPLGR ETFLCPVVWE DGWPVFAPGE
GRLPSAVAIP WATEAPRAGS WQPDDRVAGV VAPADPRWTA LRALPAEVAI AEGDVWRLPV
RAASLHDPSA PAFLGVRQQH ADVDVRCTLD VRGLAEGESA GVVLRQSERD HVAVAVTRTP
DGLRLTSERC LGGEVLRRDH GAPAEAPEVE LVVSGRGYDY AFRHAGRDLE SADGRDLDAS
ATGGFLGLWI GAYATSAGAE PSGEVRITRF AYAPAQADHG DR
//
