ID   A0A0F0L6Z9_9MICO        Unreviewed;       431 AA.
AC   A0A0F0L6Z9;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   27-MAR-2024, entry version 41.
DE   RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN   Name=gltA2 {ECO:0000313|EMBL:KJL28469.1};
GN   ORFNames=RS83_03542 {ECO:0000313|EMBL:KJL28469.1};
OS   Microbacterium oxydans.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=82380 {ECO:0000313|EMBL:KJL28469.1, ECO:0000313|Proteomes:UP000033640};
RN   [1] {ECO:0000313|EMBL:KJL28469.1, ECO:0000313|Proteomes:UP000033640}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BEL4b {ECO:0000313|EMBL:KJL28469.1,
RC   ECO:0000313|Proteomes:UP000033640};
RA   Corretto E.;
RT   "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT   metal contaminated environments.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16;
CC         Evidence={ECO:0000256|ARBA:ARBA00000308,
CC         ECO:0000256|RuleBase:RU003370};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC       ECO:0000256|RuleBase:RU003370}.
CC   -!- SIMILARITY: Belongs to the citrate synthase family.
CC       {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC       ECO:0000256|RuleBase:RU003406}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KJL28469.1}.
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DR   EMBL; JYIW01000026; KJL28469.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0F0L6Z9; -.
DR   PATRIC; fig|82380.11.peg.3569; -.
DR   OrthoDB; 9800864at2; -.
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000033640; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06114; EcCS_like; 1.
DR   Gene3D; 2.20.28.60; -; 1.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR024176; Citrate_synthase_bac-typ.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   InterPro; IPR010953; Citrate_synthase_typ-I.
DR   NCBIfam; TIGR01798; cit_synth_I; 1.
DR   PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PIRSF; PIRSF001369; Citrate_synth; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Acyltransferase {ECO:0000313|EMBL:KJL28469.1};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW   ECO:0000256|RuleBase:RU003370}.
FT   ACT_SITE        312
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT   ACT_SITE        369
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ   SEQUENCE   431 AA;  47846 MW;  6CDB6B61B02210A0 CRC64;
     MSAAADQQAT AKLTIGETTA EFPLVHGTAG NSSIDFSTLT RQTGYTGLDY GFVNTASTKS
     EITFIDGDKG ILRYRGYPIE QLANTTSYLE VAWLLIYGEL PSASELSEFD EKIRRHTLLH
     EDLKRFFSAL PHTAHPMSVL SSAVAALSTY YEGQTDPHNP EHVELNMIRM LAKLPVIAAY
     AHKKSVGQAF LYPDNSLSFV DNFLKLNFGV HSEEYEVNPV MSKALELLLI LHEDHEQNAS
     TSTVRLVGST GANQFASISA GIQALSGPLH GGANEAVLTM LGQIRDSGQS VSRFVERVKN
     KEEGVKLMGF GHRVYKNYDP RAKLVKDAAD EVLASLGVTD PLLDLAKELE ELALADDYFR
     ERRLYPNVDF YTGVIYKAMG FPTRMFTVLF AIGRLPGWLA QWRELQLDPQ TKIGRPQQLY
     TGSPERDFPT R
//