ID A0A0F3IQP4_9GAMM Unreviewed; 391 AA.
AC A0A0F3IQP4;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=3-hexulose-6-phosphate synthase {ECO:0000256|ARBA:ARBA00012890};
DE EC=4.1.2.43 {ECO:0000256|ARBA:ARBA00012890};
GN ORFNames=VZ94_01400 {ECO:0000313|EMBL:KJV07919.1};
OS Methylocucumis oryzae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylocucumis.
OX NCBI_TaxID=1632867 {ECO:0000313|EMBL:KJV07919.1, ECO:0000313|Proteomes:UP000033684};
RN [1] {ECO:0000313|Proteomes:UP000033684}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sn10-6 {ECO:0000313|Proteomes:UP000033684};
RA Pandit P.S., Pore S.D., Arora P., Kapse N.G., Dhakephalkar P.K.,
RA Rahalkar M.C.;
RT "Draft genome sequence of a novel methanotroph (Sn10-6) isolated from
RT flooded ricefield rhizosphere in India.";
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KJV07919.1, ECO:0000313|Proteomes:UP000033684}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sn10-6 {ECO:0000313|EMBL:KJV07919.1,
RC ECO:0000313|Proteomes:UP000033684};
RX PubMed=26547566; DOI=10.1007/s00248-015-0699-z;
RA Rahalkar M.C., Pandit P.S., Dhakephalkar P.K., Pore S., Arora P., Kapse N.;
RT "Genome Characteristics of a Novel Type I Methanotroph (Sn10-6) Isolated
RT from a Flooded Indian Rice Field.";
RL Microb. Ecol. 71:519-523(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose
CC 6-phosphate; Xref=Rhea:RHEA:25201, ChEBI:CHEBI:16842,
CC ChEBI:CHEBI:58121, ChEBI:CHEBI:58542; EC=4.1.2.43;
CC Evidence={ECO:0000256|ARBA:ARBA00000718};
CC -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via RuMP
CC pathway; D-fructose 6-phosphate from D-ribulose 5-phosphate and
CC formaldehyde: step 1/2. {ECO:0000256|ARBA:ARBA00005014}.
CC -!- SIMILARITY: Belongs to the HPS/KGPDC family. HPS subfamily.
CC {ECO:0000256|ARBA:ARBA00006350}.
CC -!- SIMILARITY: Belongs to the SIS family. PHI subfamily.
CC {ECO:0000256|ARBA:ARBA00009235}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJV07919.1}.
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DR EMBL; LAJX01000011; KJV07919.1; -; Genomic_DNA.
DR RefSeq; WP_045777865.1; NZ_LAJX01000011.1.
DR AlphaFoldDB; A0A0F3IQP4; -.
DR PATRIC; fig|1632867.3.peg.323; -.
DR OrthoDB; 43475at2; -.
DR UniPathway; UPA00294; UER00434.
DR Proteomes; UP000033684; Unassembled WGS sequence.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0043801; F:hexulose-6-phosphate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016853; F:isomerase activity; IEA:InterPro.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:InterPro.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:1901135; P:carbohydrate derivative metabolic process; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019647; P:formaldehyde assimilation via ribulose monophosphate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04726; KGPDC_HPS; 1.
DR CDD; cd05005; SIS_PHI; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR017553; 3-hexulose-6-phosphate_synth.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR041710; HPS/KGPDC.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR017552; PHI/rmpB.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR InterPro; IPR001347; SIS_dom.
DR InterPro; IPR046348; SIS_dom_sf.
DR NCBIfam; TIGR03128; RuMP_HxlA; 1.
DR NCBIfam; NF041755; RuMP_HxlAB; 1.
DR NCBIfam; TIGR03127; RuMP_HxlB; 1.
DR PANTHER; PTHR35039; 3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED; 1.
DR PANTHER; PTHR35039:SF3; 3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED; 1.
DR Pfam; PF00215; OMPdecase; 1.
DR Pfam; PF01380; SIS; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; Ribulose-phoshate binding barrel; 1.
DR SUPFAM; SSF53697; SIS domain; 1.
DR PROSITE; PS51464; SIS; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Reference proteome {ECO:0000313|Proteomes:UP000033684}.
FT DOMAIN 239..378
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
SQ SEQUENCE 391 AA; 41675 MW; A1C9BF0809F6D58E CRC64;
MARPLIQLAL DSLDFNQTLS LAEQTAPFVD IFEIGTPCIK HNGVELVKAL RAKFPNKLIL
VDLKTMDAGE YEATPFYAAG ADICTVLGVS GLATIKGVIK AAKAHNAEAQ IDLINVPDKL
ACAKAAAELG ANIIGVHTGL DAQAAGQTPY EDLKAISDLG LNVRISVAGG IKASTVKQVV
ATGADIIVVG AAIYGASSPA EAARTIRDAA DGKVTHQQMV VNKIRSVLSE TRDDYPSRLL
RMLDRANRVF VSGAGRSGLI CRFLGMRLMH SGYDVSMVGE IVTPSIKRGD LLIIISGSGE
TEQLIAFTKK AREVGAQILL ITAKPNSTIG SMADETMQMG TPEQFAKVQG MPMGTVFELS
TLFFLESFIS HVIHEKGIAE EEMRSRHANL E
//
