ID A0A0F3M9B0_ORITS Unreviewed; 313 AA.
AC A0A0F3M9B0;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C {ECO:0000256|ARBA:ARBA00017128};
DE EC=5.4.99.24 {ECO:0000256|ARBA:ARBA00012785};
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase {ECO:0000256|ARBA:ARBA00030705};
DE AltName: Full=rRNA pseudouridylate synthase C {ECO:0000256|ARBA:ARBA00031975};
DE AltName: Full=rRNA-uridine isomerase C {ECO:0000256|ARBA:ARBA00033053};
GN Name=rluC {ECO:0000313|EMBL:SPR05838.1};
GN ORFNames=GILLIAM_01089 {ECO:0000313|EMBL:SPR05838.1}, OTSGILL_1680
GN {ECO:0000313|EMBL:KJV52216.1};
OS Orientia tsutsugamushi str. Gilliam.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Orientia.
OX NCBI_TaxID=1359184 {ECO:0000313|EMBL:KJV52216.1, ECO:0000313|Proteomes:UP000033769};
RN [1] {ECO:0000313|EMBL:KJV52216.1, ECO:0000313|Proteomes:UP000033769}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Gilliam {ECO:0000313|EMBL:KJV52216.1,
RC ECO:0000313|Proteomes:UP000033769};
RA Daugherty S.C., Su Q., Abolude K., Beier-Sexton M., Carlyon J.A.,
RA Carter R., Day N.P., Dumler S.J., Dyachenko V., Godinez A., Kurtti T.J.,
RA Lichay M., Mullins K.E., Ott S., Pappas-Brown V., Paris D.H., Patel P.,
RA Richards A.L., Sadzewicz L., Sears K., Seidman D., Sengamalay N.,
RA Stenos J., Tallon L.J., Vincent G., Fraser C.M., Munderloh U.,
RA Dunning-Hotopp J.C.;
RT "Genome Sequencing of Rickettsiales.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:SPR05838.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Gilliam {ECO:0000313|EMBL:SPR05838.1};
RA Keele B.F.;
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000244959}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Gilliam {ECO:0000313|Proteomes:UP000244959};
RA Batty M. E., Batty M E.;
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA.
CC {ECO:0000256|ARBA:ARBA00002876}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC Evidence={ECO:0000256|ARBA:ARBA00000381};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876}.
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DR EMBL; LANO01000028; KJV52216.1; -; Genomic_DNA.
DR EMBL; LS398551; SPR05838.1; -; Genomic_DNA.
DR RefSeq; WP_047220741.1; NZ_LS398551.1.
DR PATRIC; fig|1359184.3.peg.1229; -.
DR Proteomes; UP000033769; Unassembled WGS sequence.
DR Proteomes; UP000244959; Chromosome i.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF93; PSEUDOURIDYLATE SYNTHASE RPUSD4, MITOCHONDRIAL; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000313|EMBL:SPR05838.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000244959};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 12..79
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
SQ SEQUENCE 313 AA; 35452 MW; 35CE2E5B93D27ED6 CRC64;
MKITINDCLV TTRLDKFLKR KYSHLTQGVI ERGIRKGHIK VNDAKSKAKL QVKDNDVLTI
ADYLTTCSNQ KHIKLKKKVK DNVGVKALAH KIVNDYKIAE TSDYIIINKP ALLPTQGGSK
ISLSLDDAIK YLNKSFYYDF KLVHRLDRET SGLLIIAKNH KTAIKLSQAF LHHSIEKEYT
AILQGVPSQL SGEIITNITK TGDRMVETQN GGKVSITKYQ VKAVSMNSQL SLVSFIPVTG
RTHQLRVHAA KIGHPIIGDK KYNISNTYYN YSLKLHAAKI CIPKHLFGDQ RIYYAPLPYQ
FQQVISTFFD HNQ
//
