ID A0A0F3RDJ5_ORITS Unreviewed; 304 AA.
AC A0A0F3RDJ5;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
DE Flags: Fragment;
GN Name=tilS {ECO:0000313|EMBL:KJW04530.1};
GN ORFNames=OTUT144_2168 {ECO:0000313|EMBL:KJW04530.1};
OS Orientia tsutsugamushi str. UT144.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Orientia.
OX NCBI_TaxID=1441384 {ECO:0000313|EMBL:KJW04530.1, ECO:0000313|Proteomes:UP000033580};
RN [1] {ECO:0000313|EMBL:KJW04530.1, ECO:0000313|Proteomes:UP000033580}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UT144 {ECO:0000313|EMBL:KJW04530.1,
RC ECO:0000313|Proteomes:UP000033580};
RA Daugherty S.C., Su Q., Abolude K., Beier-Sexton M., Carlyon J.A.,
RA Carter R., Day N.P., Dumler S.J., Dyachenko V., Godinez A., Kurtti T.J.,
RA Lichay M., Mullins K.E., Ott S., Pappas-Brown V., Paris D.H., Patel P.,
RA Richards A.L., Sadzewicz L., Sears K., Seidman D., Sengamalay N.,
RA Stenos J., Tallon L.J., Vincent G., Fraser C.M., Munderloh U.,
RA Dunning-Hotopp J.C.;
RT "Genome Sequencing of Rickettsiales.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJW04530.1}.
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DR EMBL; LAOR01000220; KJW04530.1; -; Genomic_DNA.
DR RefSeq; WP_052691324.1; NZ_LAOR01000220.1.
DR AlphaFoldDB; A0A0F3RDJ5; -.
DR Proteomes; UP000033580; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR NCBIfam; TIGR02432; lysidine_TilS_N; 1.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:KJW04530.1}.
FT DOMAIN 23..200
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
FT NON_TER 304
FT /evidence="ECO:0000313|EMBL:KJW04530.1"
SQ SEQUENCE 304 AA; 34927 MW; ACBA71FE9FBFBBB3 CRC64;
MNIEVNFCHN MEHFGAFEKN PKLAIAVSGG SDSLALMLLV KHWNEKVKGE ITVLTIDHHL
RSESTSEADY VSSICQNIKL QHVTLHWIHK GITGNIQAQA RKARYQLLTN YCQEHDILHL
IAGHHADDIV ENFFIRLLRG AGLTGLSSHN LFFANNVRII RPLFNITKQD LKKYLEQQNI
KWINDPSNSS NKYLRTQVRD LLKSMLISFQ HNFTVKLLKK RIMLSQMHLT RALDSVNNEI
IHYVVHAVKI YSAGFAVIDR KLFRQASPEA RYAILSYLLM IVGANTKPPR FSSLQRISLH
DIQE
//
